天龙咳喘灵胶囊防治大鼠慢性低氧性肺动脉高压的NO机制

目的:探讨天龙咳喘灵胶囊(TLC)防治大鼠慢性常压低氧性肺动脉高压的NO机制。方法:SD大鼠随机分为:正常对照组、单纯低氧组和低氧 TLC组,除正常对照组外其余两组置于常压低氧舱内饲养,10h/d,共28d,然后测定各组大鼠平均肺动脉压(mPAP)和动脉血中NO含量及NOS活性,并分析平均肺动脉压和NO含量的相关性。结果:与正常对照组相比,低氧组大鼠mPAP显著升高;TLC干预后能显著抑制低氧所致的mPAP升高,与正常对照组比较无显著性差异。慢性低氧使大鼠动脉血中NO含量和NOS活性显著降低,而低氧 TLC组与正常对照组相比,NO含量和NOS活性均无显著性差异。相关分析显示,mPAP与血浆NO含量之间呈负相关(r=0.745)。结论:TLC可通过增加NO含量,抑制大鼠慢性低氧性肺动脉高压的形成。

Effect of Tianlongkechuan capsule on release of nitric oxide in rats with chronic hypoxia-induced pulmonary hypertension

Objetive:To investigate the effect of Tianlongkechuan capsule (TLC) on chronic hypoxia-induced pulmonary hypertension and to explore its mechanism.Methods:Twenty-four Sprague-Dawley rats were randomly divided into control (C group),hypoxic (H group), and TLC groups (HT group).All these rats except those in the control group were exposed to hypoxia 8 hours each day for 4 weeks.Mean pulmonary arterial pressure (mPAP) and NO concentration and NOS activity in blood serum were detected in these rats.And the relationship between mPAP and NO concentration was analyzed.Results:The value of mPAP of H group were significantly higher than those of C and HT group(P<0.05),and plasma NO concentration and NOS activity of H group were markedly decreased than those of C and HT group(P<0.01).There was negative correlation between mPAP and NO concentration(r=0.745).Conclusion:These results indicate that TLC may have a preventive effect on chronic hypoxic pulmonary hypertension by promoting the release of NO.