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| 人白介素10逆转录病毒表达载体的构建 | |
| 引用本文： | 汪小平,赵雄飞,易绍萱,陈渝,太光平. 人白介素10逆转录病毒表达载体的构建[J]. 重庆医学, 2005, 34(6): 897-897,899 |
| 作者姓名： | 汪小平 赵雄飞 易绍萱 陈渝 太光平 |
| 作者单位： | 第三军医大学西南医院药剂科,重庆,400038;第三军医大学西南医院烧伤研究所,重庆,400038 |
| 摘 要： | 目的构建一个人白介素10(hIL-10)逆转录病毒表达载体,为研究hIL-10的生物学活性打下基础.方法用限制性内切酶消化提取质粒pCDHIL-10中hIL-10全长cDNA,采用定向克隆的方法将hIL-10基因插入逆转录病毒载体pLXSN的多克隆位点中,经转化、扩增、限制性内切酶消化,电泳鉴定分析.结果外源性hIL-10基因成功插入到逆转录病毒载体pLXSN的中.结论成功完成了人白介素10(hIL-10)逆转录病毒表达载体的构建,为今后进一步研究hIL-10的生物学活性打下了基础. |
| 关 键 词： | 白介素10 白介素10基因 逆转录病毒载体 |
| 文章编号： | 1671-8348(2005)06-0897-02 |
Construction of retroviral vector carrying human interleukin-10 |
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| WANG Xiao-ping,ZHAO Xiong-Fei,YI Shao-xuan,et al.. Construction of retroviral vector carrying human interleukin-10[J]. Chongqing Medical Journal, 2005, 34(6): 897-897,899 | |
| Authors: | WANG Xiao-ping ZHAO Xiong-Fei YI Shao-xuan et al. |
| Abstract: | Objective To study the effects of hIL-10 on graft rejection, and to set up foundation of its application in immunogene therapy. Retroviral vector carrying hIL-10 cDNA was successfully constructed.Methods To digest pCDHIL-10 with restrictive endonucleases, to isolate and purify hIL-10 complete cDNA from pCDHIL-10 and then subclone cDNA into retroviral vector pLXSN; to identify recombinant retroviral vector pLXHIL-10 by digestion with restrictive endonucleases and Agarose Gel Electrophoresis.Results Exogenous hIL-10 gene was subcloned into retroviral vector pLXSN successfully.Conclusion Retroviral vector carrying hIL-10 cDNA shall lay foundation for researching hIL-10 mechanism and its applications for curing diseases. |
| Keywords: | interleukin-10 interleukin-10 gene retroviral vector |
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