乙酰苯肼、X射线和环磷酰胺联合复制小鼠再生障碍性贫血模型

目的以乙酰苯肼、X射线、环磷酰胺联合应用的方法建立小鼠再生障碍性贫血动物模型。方法BALB/c小鼠,采用皮下注射乙酰苯肼100mg/kg,次日X射线2.0Gy照射后,于试验第5天环磷酰胺80 mg/kg腹腔注射,第15天重复以上步骤但不给予射线处理。观察试验小鼠的血象、骨髓象、造血细胞内的线粒体、造血干细胞集落的形成及肝脏、脾脏的病理变化。结果与正常对照组相比,再障模型组小鼠外周血象三系细胞均有显著降低:促红细胞生成素(EPO)含量升高;骨髓有核细胞数显著降低,造血干细胞集落的形成减低,肝脾出现再障的病理改变。结论本方法建立的再生障碍性贫血小鼠模型,简便,成功率高,外周血和骨髓的病理变化持续、稳定,符合再生障碍性贫血的临床表现。

Aplastic Anemia Mouse Model Established by Combination of Hydracetin, X-ray and Cyclophosphamide

Objective To establish a aplastic anemia mouse model by combination of hydracetin,X-ray and cyclophosphamide.Methods Hydracetin 100 mg/kg was injected subcutaneously on the back of BALB/e mice.Next day, the mice were irradiated by 2.0Gy X-ray,and the fifth day cyclophosphamide 80 mg/kg was injected into abdominal cavity. These steps were repeated the fifteenth day,but the ray treatment was not given.This experiment investigated the hemogram, bone marrow,mitochondria of hematopoietic cells,colony formation of hematopoietic stem cell,as well as the pathological changes of liver and spleen in mice.Results Compared to the control group,the peripheral hemogram of mice in the model group showed a remarkable decrease of three kinds of cells,serum Epo level was much higher,the number of nucleated cells in bone marrow and colony formation of hematopoietic stem cells exhibited significan decrease,with the pathological changes also observed in liver and spleen.Conclusion The present aplastic anemia model,being simple with high successful rate, having persistent pathological changes of peripheral blood and bone marrow,is consistent with clinical situation of aplastic anemia.