| 生物素-亲和素系统检测弓形虫病的研究 |
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| 引用本文： | 赵恒梅,周绍础,曹守箴,韩敏. 生物素-亲和素系统检测弓形虫病的研究[J]. 青岛大学医学院学报, 1997, 0(3) |
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| 作者姓名： | 赵恒梅 周绍础 曹守箴 韩敏 |
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| 作者单位： | 青岛医学院寄生虫学教研室 |
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| 摘 要： | ①目的建立简易、实用的生物素－亲和素系统（ＡＢＣ）的免疫酶染色试验（ＡＢＣ－ＩＥＳＴ）和斑点酶联免疫吸附试验（ＡＢＣ－Ｄｏｔ－ＥＬＩＳＡ）的方法，以检测弓形虫病的微量抗体。②方法以全虫和可溶性虫体为抗原，在常规ＩＥＳＴ和Ｄｏｔ－ＥＬＩＳＡ的基础上，加用ＡＢＣ，观察其特异度和敏感度。③结果ＡＢＣ－ＩＥＳＴ和ＡＢＣ－Ｄｏｔ－ＥＬＩＳＡ的特异度和常规法相同，但敏感度有显著提高。④结论ＡＢＣ法可应用于微量抗体及早期感染的检测。
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| 关 键 词： | 弓形虫病；生物素；免疫酶技术；酶联免疫吸附测定 |
STUDY ON DIAGNOSIS OF TOXOPLASMOSIS WITH AVIDIN BIOTIN SYSTEM |
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| Zhao Hengmei,Zhou Shaochu,Cao Shouzhen,et al. STUDY ON DIAGNOSIS OF TOXOPLASMOSIS WITH AVIDIN BIOTIN SYSTEM[J]. Acta Academiae Medicinae Qingdao Universitatis, 1997, 0(3) |
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| Authors: | Zhao Hengmei Zhou Shaochu Cao Shouzhen et al |
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| Affiliation: | Zhao Hengmei,Zhou Shaochu,Cao Shouzhen,et al Department of Parasitology,Qingdo Medical College,Qingdao 266021 |
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| Abstract: | Objective To establish simple and practical ABC IEST and ABC DOT ELISA methods for detecting the trace amount of toxoplasma antibody. Methods ABC method together with rotine IEST and Dot ELISA was used to detect the whole protozoa body and soluble extract. Results The specificity of ABC IEST and ABC Dot ELISA were the same as the rotine method, but the sensitivity were 2 and 2.4 times higher respectively than that of the rotine method. Conclusion The results of this study suggest that both ABC IEST and ABC Dot ELISA methods may be available in the detection of trace amount of toxoplasma antibody and early diagnosis of toxoplasmosis infections. |
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| Keywords: | toxoplasmosis biotin immunoenzyme techniques enzyme linked immunosorbent |
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