曲古菌素A对Molt-4细胞细胞周期阻滞和凋亡的作用

目的:本研究旨在探讨曲古菌素A(TSA)对Molt-4细胞的诱导细胞周期阻滞及凋亡的作用及TSA高效低毒的抗肿瘤特性。方法:应用细胞培养、PI单标流式细胞术和DNA Ladder等方法初步探讨TSA对Molt-4细胞的诱导细胞周期阻滞及凋亡的作用。结果:在一定浓度范围内,TSA能以浓度依赖性诱导Molt-4细胞S期及G2期阻滞。TSA以浓度依赖性方式诱导Molt-4细胞凋亡,随着TSA的浓度的升高,细胞凋亡率也显著增高。TSA的各实验组的凋亡率和对照组的凋亡率相比均有显著性差异,P<0.01。经200 nmol/L的TSA处理后,Molt-4细胞凋亡率增高,且呈时间依赖性效应关系。TSA在显著诱导Molt-4细胞凋亡的浓度范围内对正常人外周血单个核细胞(NPBMNC)无明显的诱导凋亡作用。结论:TSA有明确的诱导Molt-4细胞周期阻滞和诱导Molt-4细胞凋亡的能力,并且这种作用呈浓度和时间依赖性,这可能是TSA体外抑制白血病细胞系Molt-4生长和发挥抗白血病作用的主要机理之一,与NPBMNC相比较,TSA能够选择性诱导Molt-4细胞凋亡。

Effects of Trichostatin A on Cell Cycle Arrest and Apoptosis in Molt-4 Cells

Objective: To explore the effects of trichostatin A(TSA) on cell cycle and apoptosis in Molt-4 cells and the anti-tumoral actions.Methods: The effects of TSA on cell cycle arrest and apoptosis in Molt-4 cells were examined using cell culture,PI staining,flow cytometry and DNA Ladder.Results: Within a certain range of dose,TSA induced S phase arrest and G_2 phase arrest in Molt-4 cells in a dose-dependent manner.The apoptosis induction effect of TSA in Molt-4 cells was also dose-dependent.The apoptosis percentage increased according,with the increase of the concentration of TSA.The apoptosis percentages of the experiment groups had significant difference from the control group(P<0.01).The apoptosis induction effects of TSA in Molt-4 cells were time-dependent under the treatment of 200 nmol/L TSA.Besides,TSA showing significant apoptotic cytotoxicity in Molt-4 cells did not demonstrate apparent apoptosis induction in normal human peripheral blood mononuclear cells(NPBMNC) with the definite dose range.Conclusion: TSA shows effects of definite and significant cell cycle arrest and apoptosis induction on Molt-4 cells in time-and dose-dependent manners.TSA is able to selectively induce apoptosis in Molt-4 cells with low toxicity in NPBMNC at the same time.