大豆提取物对去卵巢大鼠能量平衡的影响

背景:去卵巢动物多用于女性骨质疏松的研究模型,但少有研究去卵巢对动物能量平衡的影响。以前研究大豆异黄酮可降低高脂饲料引起的大鼠高脂血症,大豆异黄酮对去卵巢动物的能量代谢如何?目的:探讨大豆提取物及其有效成分对去卵巢大鼠能量代谢的药理作用,为2型糖尿病、脂代谢紊乱、高血压和冠状动脉粥样硬化性心脏病的一级康复预防提供理论依据。设计:以实验动物为研究对象的随机对照实验研究。单位:一所大学的细胞与生物化学实验室。材料:Wistar大鼠90只(SFP级,合格证号:scxk11-00-006)随机分成正常组、假手术组、模型组、雌激素组、高剂量大豆黄酮组、低剂量大豆黄酮组、高剂量大豆提取物组、低剂量大豆提取物组、大豆多糖组,每组10只。干预:除假手术组与正常组外,其余各组均切除双侧卵巢。术后1周开始每周测量一次体质量、日进食量。6周后处死动物,计算饲料转化率,测量身长,计算体质量系数,分离腹部脂肪并称重。主要观察指标:①大豆提取物对去卵巢大鼠腹部脂肪堆积的作用。②大豆提取物对去卵巢大鼠进食量的影响。③大豆提取物对去卵巢大鼠饲料转化率的作用。结果:切除卵巢后动物进食量、体质量与体质量系数增加、饲料转化率加大,腹部脂肪堆积。雌激素组、大豆提取物组及大豆黄酮组均不同程度减弱去卵巢引起的能量代谢改变,但大豆多糖无作用。结论:去卵巢大鼠可作为更年期妇女肥胖的动物模型,大豆提取物有减弱去卵巢引起的体质量增加、进食和饲料转化率增加等病理改变,其有效成分为黄酮类化合物。

Effects of soy extract on energy balance in ovariectomized rats

BACKGROUND: Ovariectomized animals usually are employed for the study of women osteoporosis while little is known about theenergy balance of ovariectomized animals. Many previous studies showed that soy isoflavone could decrease the hyperlipemia resulted by high-fat feed, and how about the effect of soy isoflavone on the energy metabolism in ovariectomized animals?OBJECTIVE: To explore the pathologic effect of soy extract and its active components on the energy metabolism in ovariectomized rats so as to provide adequate evidences for the primary rehabilitation and prevention of type Ⅱ diabetes mellitus, disturbance of lipid metabolism as well as hypertension and coronary atherosclerotic heart disease.DESIGN: A randomized and controlled trial with the experimental animals as subjects.SETTING: Laboratory of Cell and Biochemistry in a university.MATERIALS: Ninety Wistar rats (SFP grade, license code: scxk11-00-006) were randomized into 9 groups: normal group, sham group,model group, estrogen group, high-dose soy flavone group, low-dose soy flavone group, high-dose soy extract group, low-dose soy extract group and soy polysaccharide group, with 10 rats in each group.INTERVENTION: Except the rats in normal group and sham group,the bilateral ovaries of other rats were all removed. From one week after operation, body weight and daily food intake were detected once a week.Six weeks later, the rats were killed to calculate the forage transformation efficiency, measure body length, work out the body mass index (BMI),and separate the abdominal fat and weight.MAIN OUTCOME MEASURES: ①Effect of soy extract on the abdominal fat accumulation of ovariectomized rats. ②Effect of soy extract on food intake of ovariectomized rats. ③Effect of soy extract on forage transformation efficiency of ovariectomized rats.RESULTS: After ovariectomy, the food intake, body weight and BMI all raised, and the forage transformation efficiency increased, with abdominal fat accumulation. The changes of energy metabolism induced by ovariectomy were all weakened at different degrees in estrogen group, soy extract group and soy flavone group, while no influenced was found in soy polysaccharide group.CONCLUSION: The ovariectomized rats can be used as animal model of the climacteric fat women. The soy extract, with the effective component of flavonoid, can reduce the pathologic changes such as the increase of food intake, body weight and forage transformation efficiency induced by ovariectomy.