Tributyltin-binding protein type 1 has a distinctive lipocalin-like structure and is involved in the excretion of tributyltin in Japanese flounder,Paralichthys olivaceus |
| |
| Affiliation: | 1. Institute of Chemistry, University of Silesia, 9 Szkolna Street, 40-006 Katowice, Poland;2. Faculty of Computer Science and Information Technology, West Pomeranian University of Technology, 49 Zolnierska Street, 71-210 Szczecin, Poland;3. Polcargo International, Poboznego Street, 70-900 Szczecin, Poland;1. Department of Neuroscience, Uppsala University, 75124 Uppsala, Sweden;2. Department of Biology, Norwegian University of Science and Technology, 7491 Trondheim, Norway;3. Marine and Freshwater Research Institute, 101 Reykjavik, Iceland;4. Department of Chemistry, Umeå University, 90187 Umeå, Sweden;5. Department of Ecology and Environmental Science, Umeå University, 90187 Umeå, Sweden |
| |
| Abstract: | Tributyltin-binding protein type 1 (TBT-bp1) is a newly discovered protein that binds with TBT in the blood of the Japanese flounder, Paralichthys olivaceus. We determined the genomic sequence of TBT-bp1 and found that this protein has a conserved exon–intron structure that is common to the lipocalin protein family. The secondary and tertiary structures of TBT-bp1, predicted from amino acid sequence, included at least two α-helices and eight β-sheets that are conserved in all lipocalins and form a barrel structure that may bind with ligands. Analysis of the gene structure, secondary structure, and tertiary structure demonstrated that TBT-bp1 could be classified as a lipocalin. A homology search revealed the presence of TBT-bp1-like proteins in eight species of teleost. When flounder were injected intraperitoneally with TBT-d27 at 11.6 μg/fish, TBT-d27 was detected in the blood and in the skin mucus. The concentration of TBT-d27 in mucus was approximately 1/100 of that in the serum. Western blotting analysis revealed that TBT-bp1 was present in the skin mucus. These results suggest that TBT-bp1 in Japanese flounder binds with TBT and is excreted from the body via the mucus. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
