电针对抑郁模型大鼠行为活动的影响

目的:观察电针及合用小剂量三环类抗抑郁药———氯丙咪嗪后抑郁模型大鼠行为活动的改善情况,探讨电针及针药结合的抗抑郁效果。方法:SD大鼠48只,分为正常组、模型组、电针组、小剂量氯丙咪嗪(2.5 mg/kg)组、大剂量氯丙咪嗪(5 mg/kg)组、电针合用小剂量氯丙咪嗪(2.5 mg/kg)组。采用国际通用的不可预知的长期应激刺激制造大鼠抑郁模型。应激刺激14 d后,治疗组(包括电针组和不同剂量的药物组)在给予应激刺激的同时开始治疗。药物治疗每天1次,共给药14次。电针取“百会”、右侧“阳陵泉”,疏密波(疏波频率4 Hz,串长2.5 s;密波频率60 Hz,串长5 s),强度≤1 mA,持续30 min/次,隔天1次,共治疗7次。采用活动性测试和强迫游泳实验评判大鼠抑郁程度的改善情况。结果:造模成功后,大鼠在活动性测试中垂直和水平运动显著下降,强迫游泳中静止时间显著增加。电针和大剂量的氯丙咪嗪(5 mg/kg)均可以增加大鼠的垂直和水平运动,减少强迫游泳实验中大鼠的静止时间。电针与小剂量氯丙咪嗪合用可进一步减少静止时间,增加垂直运动和水平运动。结论:电针具有良好的抗抑郁效果,小剂量药物合用电针一方面可降低用药剂量,另一方面可进一步提高电针疗效。

Effect of Electroacupuncture on Behavioral Activities of Depression Rats

Objective: To observe the effect of electroacupuncture(EA) combined with Clomipramine(Clo) on behavioral activities of depression rats.Methods: Forty-eight SD rats were randomized into normal control,model,EA,Clo(2.5 mg),Clo(5 mg) and EA+Clo(2.5 mg) groups,with 8 cases in each group.Chronic unpredictable stress(CUS) model was established by forcing the rat to have fasting,cool-water swim,water-deprivation,high temperature environment,etc.in 28 days.EA((4 Hz) and (60 Hz),duration of the pulse train:(2.5 s) and(5 s),≤(1 mA)) was applied to "Baihui"(GV 20) and right "Yanglingquan"(GB 34) for(30 min), once every other day,7 times altogether.CHP was given to the animals by intraperitoneal injection.The open-field test was used to observe the rats' rearing and crossing activities,and the forced swim test was employed to determine the rats' duration of immovability(DI) during(5 min) in a water pool.Results: In comparison with normal control group,the rearing and crossing movement times of rats in model group decreased significantly(P<0.05);while compared with model group,the rearing times of rats in EA,Clo(2.5 mg),Clo(5 mg) and EA+Clo(2.5 mg) groups,and crossing movement times of rats in EA,Clo(5 mg) and EA+Clo(2.5 mg) groups increased remarkably(P<0.05),and the effect of EA+Clo(2.5 mg) was significantly superior to those of EA and Clo(2.5 mg) groups in the rearing times and to that of EA group in the crossing times(P<0.05).In comparison with normal control group,the rats' DI in the water pool in model group prolonged significantly(P<0.05);while compared with model group,the DI of EA,Clo(5 mg) and EA+Clo(2.5 mg) shortened significantly(P<0.05),and the effect of EA+Clo(2.5 mg) group was significantly superior to that of EA group on the 28~(th) day(P<0.05).Conclusion: EA works well in relieving