HPLC-DAD同时测定山茱萸饮片中5个成分的含量

 目的采用高效液相二极管阵列检测法(HPLC-DAD)同时测定4个主要产地的山茱萸饮片中5个成分的含量:没食子酸、5-羟甲基糠醛(5-HMF)、莫诺苷、獐牙菜苷、马钱素。方法应用Zorbax C₁₈柱,以0.1%磷酸溶液和0.1%磷酸乙腈溶液为流动相,梯度洗脱,流速1 mL·min^-1,柱温30℃,检测波长218,240和284 nm。结果5个成分的峰面积与浓度的线性关系良好(r>0.999 6);加样回收率为98.16%～102.53%。结论该方法简单、准确、重现性好,可为全面评价和控制山茱萸饮片质量提供依据。

Simultaneous Determination of Five Active Constituents in Cornus officinalis by HPLC-DAD

OBJECTIVE To establish a high performance liquid chromatographic method with diode array detection(HPLC-DAD) for simultaneous determination of five active constituents in Cornus officinalis from four main place of product.METHODS Five constituents were analyzed simultaneously with a Zorbax C₁₈ column by gradient elution using 0.1% phosphoric acid water-solution-0.1% phosphoric acid acetonitrilesolution as the mobile phase.The column was maintained at 30 ℃.The flow rate was 1 mL·min^-1.The detection wavelength was set at 218,240 and 284 nm.RESULTS All the constituents showed good linearity(r>0.999 6) in the range of the tested concentration,the average recoveries of the method were between 98.16% and 102.53%.CONCLUSION The validated method has the advantages of simplicity,precision and reliability,allowing its intrinsic quality control of Cornus officinalis.