二氧化氯与戊二醛对乙型肝炎表面抗原破坏效果的分析

目的 对二氧化氯（ClO2破坏乙型肝炎表面抗原（HBsAg)抗原性的效果与戊二醛进行比较研究。方法 采用酶联免疫吸附实验（ELISA）进行检测。结果 ClO2消毒效果较戊二醛好，浓度40mg/L时可将HBsAg抗原性破坏，戊二醛浓度则需为200mg/L;pH值对ClO2和戊二醛的消毒效果有影响。在酸性环境中（pH值6.0时）。ClO2的破坏效果最佳。碱性戊二醛则在碱性环境中（pH值为8.0时）效果最强；20%小牛血清即对ClO2的消毒效果有明显影响，而50%以上的小牛血清才对戊二醛有影响。结论。ClO2是一种快速、高效的消毒剂。

Effects of Chlorine Dioxide and Glutaraldehyde on Inactivation of Hepatitis B Virus Surface Antigen:A Comparative Analysis

OBJECTIVE To study the disinfection effects of chlorine dioxide(ClO 2 )on inactivation of hepatitis B virus surface antigen(HBsAg),and to compare with those of glutaraldehyde.METHODS The neutralizer selection test and HBsAg destruction test were applied.RESULTS Results showed that the effects of ClO 2 on inactivation of HBsAg were better than those of glutaraldehyde. Comparing with 200 mg/L glutaraldehyde, only 40 mg/L with a 20 min contact time could destroy HBsAg antigenicity. Different pH values would affect the inactivation effects of ClO 2 and glutaraldehyde: ClO 2 had the best effect at pH=6.0,but glutaraldehyde did at pH=8.Comparing with 50% glutaraldehyde, only 20% calf serum had obvious effects on the disinfection effects of ClO 2 .CONCLUSIONS This study indicated that ClO 2 was an effective,rapid disinfectant.