生物发光成像对裸鼠肺部转移瘤的早期检测研究

目的:探讨生物发光成像早期监测裸鼠肺部转移瘤及定量评估肿瘤大小的价值?方法:利用生物发光成像检测含荧光素酶报告基因的人肺腺癌SPC-A1-luc细胞在体内外的生物发光活性?经尾静脉注射SPC-A1-luc细胞建立裸鼠肺部转移瘤模型,应用生物发光成像实时监测肺部转移瘤的生长状态并定量评估肺部肿瘤大小,同时用micro-CT检测肺部转移瘤?结果:SPC-A1-luc细胞能高水平并稳定表达荧光素酶,生物发光光子数与细胞量呈良好的线性相关(R2=0.996 6),且与皮下肿瘤体积呈良好的直线相关(R2=0.984 9)?生物发光成像监测裸鼠肺部转移瘤,在第1周即可检测到肺部弥漫分布的肿瘤细胞,第3周肺部生物发光信号降至最低,定量分析表明此时肺部约有600个肿瘤细胞?而第3周micro-CT未能检测到肺部肿瘤,直到第6周micro-CT才检测到肺部肿瘤?结论:利用生物发光成像能早期监测肺部转移瘤的生长并定量分析肿瘤大小,其灵敏度高于micro-CT成像?

Application of bioluminescence imaging on early intrapulmonary micrometastases tumors in mouse model

Objective:To investigate the ability of bioluminescence imaging (BLI)used for early detection of lung tumors and quantitative measurement of tumor size. Methods:The luciferase activity of SPC-A1-luc cell was detected in vitro by BLI. SPC-A1-luc cells were subcutaneously injected into nude mice to establish mice subcutaneous tumor model,and the subcutaneous tumor was imaged every week by BLI and measured by caliper. SPC-A1-luc cells were intravenously injected into nude mice to establish intrapulmonary micrometastases model,BLI and micro-CT were used to monitor tumor growth and quantitatively analyze tumor size. Results:SPC-A1-luc cells stably and highly expressed luciferase,and the luciferase activity was significantly correlated with cell numbers(R2=0.9966)and tumor volume(R2=0.9849). Bioluminescence signals were diffused distribution in bilateral lung at the first week. On the third week,quantitative analysis showed that bioluminescence signals were the lowest and approximately 600 tumor cells were growing in the lungs. However,intrapulmonary micrometastases could not be detected by micro-CT until the 6th week, it was three weeks later than BLI. Conclusion:Intrapulmonary micrometastases could be early detected and quantitatively analyzed by BLI,which has higher sensitivity than micro-CT.