| Caco-2细胞对酸枣仁皂苷A的跨膜转运 |
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| 引用本文： | 章新晶,熊淑华,邹霞. Caco-2细胞对酸枣仁皂苷A的跨膜转运[J]. 江西医药, 2011, 46(1): 8-10 |
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| 作者姓名： | 章新晶 熊淑华 邹霞 |
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| 作者单位： | 南昌大学第二附属医院药剂科,南昌,330006 |
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| 基金项目： | 江西省卫生厅中医处课题,编号2007A119 |
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| 摘 要： | 目的研究酸枣仁皂苷A（Jujuboside A,JuA）在Caco-2细胞的跨膜转运特性。方法采用体外培养的Caco-2细胞单层模型,考察时间、介质pH值、药物浓度、抑制剂对JuA在Caco-2细胞上转运的影响。结果 Caco-2细胞转运JuA呈时间及浓度依赖性;在pH为5.0～8.0范围内,Caco-2细胞对JuA的转运不受pH值的影响;P-糖蛋白（P-glycoprotein,P-gp）抑制剂维拉帕米（Verapamil,Ver）对Caco-2细胞转运JuA无影响;线粒体呼吸链复合体Ⅳ抑制剂叠氮化钠（Sodium azide）对Caco-2细胞转运JuA有抑制作用;JuA的AP-BL侧的Papp与BL-AP侧的Papp的两组均数比较无统计学意义。结论 JuA不是P-gp的底物,其跨膜转运是被动转运与主动转运共同参与的过程。
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| 关 键 词： | 酸枣仁皂苷A Caco-2细胞 P-糖蛋白 跨膜转运 高效液相 |
Transport of Jujuboside A across Caco-2 cell model |
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| Zhang Xinjing,Xiong Shuhua,Zou Xia. Transport of Jujuboside A across Caco-2 cell model[J]. Jiangxi Medical Journal, 2011, 46(1): 8-10 |
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| Authors: | Zhang Xinjing Xiong Shuhua Zou Xia |
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| Affiliation: | Zhang Xinjing,Xiong Shuhua,Zou Xia.Department of pharmacy,the second affiliated hospital of Nanchang university,Nanchang,330006,China |
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| Abstract: | Objective To study the transport characteristics of jujuboside A across Caco-2 cell model.Methods Caco-2 cell model was used in vitro to investigate the influence of time,pH,drug concentration,inhibitors on the transport of jujuboside A.Results JuA transporting across Caco-2 cell monolayers has dependence on time and drug concentration,there had no influence on the transport of jujuboside A from 5.0 to 8.0 pH,the inhibitor of P-gp,had no influence on JuA across Caco-2 cell,but Sodium azide the inhibitor of ... |
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| Keywords: | Jujuboside A Caco-2 cell P-glycoprotein transepithelial transport HPLC |
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