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| HPLC测定复方吴茱萸软膏中吴茱萸碱和吴茱萸次碱的含量 | |
| 引用本文： | 周雪红,李志浩. HPLC测定复方吴茱萸软膏中吴茱萸碱和吴茱萸次碱的含量[J]. 国际中医中药杂志, 2011, 33(12): 1101-1103. DOI: 10.3760/cma.j.issn.1673-4246.2011.12.017 |
| 作者姓名： | 周雪红 李志浩 |
| 作者单位： | 湖北医药学院附属东风医院药剂科,十堰,442008 |
| 摘 要： | 目的 建立同时测定复方吴茱萸软膏中吴茱萸碱和吴茱萸次碱含量的方法.方法 采用HPLC法,色谱柱为Agilent HC-C18(250 mm×4.6 mm,5μm),流动相为乙腈-0.08％辛烷磺酸钠(41∶59),流速为0.8 ml/min,柱温30℃,检测波长为225 nm.结果 吴茱萸碱和吴茱萸次碱分别在0.6... |
| 关 键 词： | 吴茱萸碱 吴茱萸次碱 复方吴茱萸软膏 高压液相色谱法 |
Simultaneous determination of evodiamine and rutaecarpine of compound Wuzhuyu ointment by HPLC |
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| ZHOU Xue-hong,LI Zhi-hao. Simultaneous determination of evodiamine and rutaecarpine of compound Wuzhuyu ointment by HPLC[J]. International Journal of Traditional Chinese Medicine, 2011, 33(12): 1101-1103. DOI: 10.3760/cma.j.issn.1673-4246.2011.12.017 | |
| Authors: | ZHOU Xue-hong LI Zhi-hao |
| Abstract: | Objective To establish a method for the determination of evodiamine and rutaecarpine of compound wuzhuyu ointment.Methods HPLC method was used with Agilent HC-C18(250 mm× 4.6 mm,5 μm) column and the mobile phase being acetonitrile-0.08％ ocane sutfonic acid sodium(41:59).The flow rate was 0.8 ml/min,the column temperature was 30℃,and the UV detector was set at 225 nm.Results The linear ranges of evodiamine and rutaecarpine were 0.62～ 12.40 μg/ml (r=0.9997) and 0.24～4.8 0 μg/ml (r=0.9996),respectively.The average recovery of evodiamine and rutaecarpine was 97.9％ and 98.0％,respectively.Conclusion The method is simple,repeatable and accurate.It can be applied in quantitative determination of evodiamine and rutaecarpine in compound Wuzhuyu ointment. |
| Keywords: | Evodiamine Rutaecarpine Compound Wuzhuyuointment HPLC |
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