反相高效液相色谱法测定人血清中奥氮平浓度

目的建立测定血清中奥氮平浓度的反相高效液相色谱（RP-HPLC）法。方法血清碱化后用乙醚提取，采用RP-HPLC法检测，色谱柱为Hypersil ODS柱（150mm×4．6mm，2．5μm），流动相为磷酸盐缓冲液（pH=6．86，内含0．5％三乙胺）-甲醇（40：60，V／V），流速为1．0mL／min，检测波长为270nm，柱温25℃。结果血清中奥氮平质量浓度线性范围为10．16～203．00μg／L（r=0．9998），最低检测质量浓度为1．22μg／L，相对回收率为99．4％～100．6％，日内RSD为2．00％～2．29％（n=5），日内RSD为2．05％～4．28％（n=5）。结论该方法简便、准确，重现性好，灵敏度高，适用于奥氮平血药浓度的监测。

Determination of Olanzapine Concentration in Serum by RP- HPLC

Objective To develop a RP- HPLC method for the determination of olanzapine concentration in serum. Methods The alkalified serum samples were extracted with diethyl ether, the separation was made by using a reversed-phase Hypersil ODS column with isocratic mobile phase consisting of phosphat buffer （pH = 6.86, containing 0, 5% triethylamine） - methanol （60 ： 40, V/V） ;the flow rate was 1.0 mL/min and the detective wavelength was 270 nm. The temperature of the column was 25℃. Results The linear range was 10. 16- 203.00μg/L （r=0.999 8）, the lowest detectable concentration in serum was 1.22μg/L. The recovery rates were 99.39%- 100.59%.The intra-day RSD was 2.00% - 2.29%. The inter- day RSD was 2.05% -4.28%. Conclusion The established method is sensitive, precise, reproducible, and suitable for the TDM of olanzapine.