| 反相高效液相色谱法测定中黄胶囊中葛根素的含量 |
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| 引用本文： | 童荣生,席庆,孙世明,吴正中. 反相高效液相色谱法测定中黄胶囊中葛根素的含量[J]. 中国药房, 2002, 13(6): 365-366 |
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| 作者姓名： | 童荣生 席庆 孙世明 吴正中 |
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| 作者单位： | 1. 四川省人民医院药剂科,成都市,610072
2. 华西医科大学药学院 |
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| 摘 要： | 目的 :建立中黄胶囊中葛根素的含量测定方法 ,以控制该产品的质量。方法 :采用反相高效液相色谱法 ,以DiamonsilC18 为固定相的色谱柱 ,甲醇 -0 015 %磷酸溶液 (23∶77)为流动相 ,检测波长249nm。结果 :葛根素的进样量在0 020μg～0 120μg 范围内与其峰面积有良好的线性关系 (r=0 9999) ,平均加样回收率为98 54 % ,RSD为1 07 %。结论 :该方法简便易行、准确可靠 ,可用于中黄胶囊的质量控制
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| 关 键 词： | 葛根素 中黄胶囊 反相高效液相色谱法 含量测定 |
| 文章编号： | 1001-0408(2002)06-0365-02 |
| 修稿时间： | 2001-07-09 |
Determination of the Content of Puerarin in Zhonghuang Capsules With RP- HPLC |
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| TONG Rongsheng,XI Qing,SUN Shiming,WU Zhengzhong. Determination of the Content of Puerarin in Zhonghuang Capsules With RP- HPLC[J]. China Pharmacy, 2002, 13(6): 365-366 |
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| Authors: | TONG Rongsheng XI Qing SUN Shiming WU Zhengzhong |
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| Abstract: | OBJECTIVE:To establish a RP-HPLC method for the determination of the content of puerarin in Zhonghuang capsules to control the quality of the product METHODS:The determination was performed on a Diamonsil C18 column(5μm,150mm×4 6mm) by UV detector at 249nm with methanol-0 015% phosphorate acid(23∶77) as the mobile phase at a flow rate of 1 2ml/min RESULTS:The calibration cure was linear in the range from 0 020μg to 0 120μg of puerarin(r=0 9 999) The average recovery and the relative standard deviation of puerarin were 98 54% and 1 07%(n=6),respectively CONCLUSION:The method is simple,accurate and reliable and can be used for the quality control of Zhonghuang capsules |
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| Keywords: | puerarin Zhonghuang capsule HPLC determination of content |
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