不同佐剂对旋毛虫Ts87重组蛋白免疫小鼠保护性的影响

目的 比较福氏完全佐剂（CFA）等6种佐剂对于旋毛虫Ts87重组蛋白（rTs87）免疫保护性的影响。 方法 经十二烷基磺酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和蛋白质印迹（Western blotting）分析鉴定的rTs87分别与上述各种佐剂免疫ICR小鼠，每间隔2周免疫1次，共免疫3次。ELISA检测免疫小鼠血中抗原特异性IgG抗体水平。攻击感染45 d后剖杀小鼠取肌幼虫（ML）并计数，计算肌幼虫减虫率。 结果 rTs87相对分子质量（Mr）约为40 000，可被旋毛虫感染鼠血清和rTs87免疫鼠血清识别，分别与佐剂CFA（或IFA）、IMS 1312、ISA720、Quil-A及氢氧化铝免疫后均引起较强的IgG抗体反应，各佐剂免疫组减虫率分别为49.4%、49.2%、63.5%、65.1%和70.8%，与空白对照组比较有统计学意义；单纯抗原rTs87免疫组获得了23.7%的减虫率，与对照组及各佐剂免疫组差异均有统计学意义（P<0.05）。结论 佐剂CFA（或IFA)、IMS1312、ISA720、Quil-A及氢氧化铝均能不同程度增强rTs87对小鼠的保护性免疫力，其中佐剂ISA720、Quil-A和氢氧化铝的保护效果更好。

Effect of Different Adjuvant Formulations on the Induced Protection of Mice Immunized With Recombinant Protein Ts87 of Trichinella spiralis

Objective To study the effect of 6 formulations of adjuvants on immunoprotection in mice induced by the recombinant Ts87 protein (rTs87) of Trichinella spiralis. Methods ICR mice were vaccinated subcutaneously 3 times in 2-week intervals with rTs87 and different adjuvants CFA or IFA, Montanide IMS1312, Montanide ISA720, Quil-A and A1(OH)3, respectively. The antibody response was detected by ELISA and the purified rTs87 was analyzed by SDS-PAGE and Western blot. The protection induced in mice was then evaluated by muscle larvae reduction after Trichinella spiralis challenge. Results The results clearly showed that all the 6 adjuvants were able to enhance specific anti-rTs87 IgG response. SDS-PAGE and Western blot revealed that the molecular weight of expressed protein was around Mr 40 000 and could be recognized by sera from mice infected with Trichinella spiralis and mice immunized with rTs87. The reduction rate of muscle larvae was 49.4%, 49.2%, 63.5%, 65.1% and 70.8%, respectively. Conclusion Protective immunity of rTs87 can be enhanced by adjuvant CFA or IFA, IMS1312, ISA720, Quil-A and Al(OH)3, and better effect is observed with the latter 3 adjuvants.