不同程度心理应激对小鼠卵巢内氧化应激水平的影响

目的:探讨不同程度刺激对小鼠卵巢氧化应激水平的影响,以期为不孕症患者辅助心理治疗提供理论依据。方法:80只雌鼠随机分为4组,给予不同程度且不可预知性刺激4周,观察小鼠体质量及行为学改变。造模成功后,检测小鼠血清中超氧化物歧化酶(SOD)含量、活性氧(ROS)水平,小鼠卵巢组织中SOD活性、丙二醛(MDA)含量、SOD2、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)3种抗氧化物酶的m RNA水平。结果:不同程度心理应激组与正常组相比,小鼠体质量降低,不动时间和中央区域滞留时间延长,进入中央区域次数及中央区域活动时间减少,差异有统计学意义(P<0.05);血清中ROS水平升高,SOD含量降低,卵巢组织中SOD活性降低,MDA含量升高,SOD2、CAT及GSH-Px活性降低,差异有统计学意义(P<0.05)。结论:心理应激能够降低小鼠的自主活动欲望和探索欲望,ROS的累积引发机体内氧化应激反应,影响女性生殖功能。

Effects of Psychological Stress on Ovarian Oxidative Stress in Mice

Objective: To observe the the ovarian oxidative stress in mice undergoing different degrees of stimulation, as a theoretical reference for adjuvant psychotherapy of female infertility. Methods:Eighty female mice were randomly divided into 4 groups. The unpredictable stimulation at different degrees was given for 4 weeks continuously. The body weight and behavioral changes were observed to confirm whether the model of psychological stress was successfully prepared. The levels of serum SOD and ROS, the ovarian SOD activity and MDA, and the levels of ovarian SOD2, CAT and GSH-Px mRNAs were detected. Results:The body weight was decreasedin the psychological stress groups when compared with the control group, the immobility time and the central area retention time were prolonged, and the number of times entering the central area and the central area activity time were decreased. The level of serum ROS was increased, while the level of serum SOD was decreased.In the ovarian tissues, the activity of SOD was decreased and the level of MDA increased, while the activities of SOD2, CAT and GSH-Pxwere decreased. Conclusions:Psychological stress can reduce the desire for independent activities and exploration. The ROS accumulation can lead to oxidative stress and affect female reproductive function.