甘草甜素对人颊黏膜成纤维细胞炎症反应的初步研究

目的：观察甘草甜素对经脂多糖刺激后体外培养的人颊黏膜成纤维细胞炎症反应的作用。方法：取正常人颊黏膜，组织块法体外培养颊黏膜成纤维细胞，加入不同浓度的甘草甜素，培养24h、48h、72h后，经MTT法检测每孔的光密度值（OD）。用脂多糖（LPS）对细胞进行干预，用来模拟口腔扁平苔藓（0LP）的炎症状态。以第4代成纤维细胞为对照组、加入脂多糖为炎症组、同时加入脂多糖和甘草甜素为实验组，用ELISA法分别检测三组白细胞介素-6（IL-6）的浓度。结果：MTT法测出甘草甜素在一定范围内浓度越高、时间越长，抑制细胞的增殖作用就越强；ELISA法检测出炎症组较对照组上清液中IL-6浓度明显升高，实验组较炎症组上清液中IL-6浓度明显降低。结论：甘草甜素可明显抑制由脂多糖刺激的人颊黏膜成纤维细胞分泌IL-6。甘草甜素在治疗OLP的炎症反应中有重要作用。

Glycyrrhizin＇s preliminary exploration on the inflammatory reaction of human oral buccal mucosa fibroblasts.

Objective： Observe the effect of glycyrrhizin on inflammatory reaction of human buccal mucosal fibroblasts （HBMFs）which is stimulated by lipopolysaccharide. Method：Take normal human oral buccal mucosa,then culture the buccal mucosal fibroblasts in vitro with the tissue block method. Adding different concentrations of glycyrrhizin and cultured for 24 h, 48 h, 72 h, after that assay every pore of the OD by MTT method. Use lipopolysaccharide （LPS） intervene cells to simulate inflammatory state of oral lichen planus （OLP）. The fourth generation of fibroblasts is control group. Adding LPS is inflammatory group, while adding LPS and glycyrrhizin is experimental group. Using the method of ELISA to measure the interleukin 6 （IL-6） concentration in the three groups. Result： The MTT method tells us that glycyrrhizin which has higher concentration and longer time on cells have stronger effect on inhibits cell proliferation. ELISA detected that Inflammation group have higher interleukin 6 concentration than control group in the supernatant. The experimental group have lower in- terleukin 6 concentration than inflammation group in the supernatant. Conclusion： Glycyrrhizin can significantly inhibited IL-6＇s secretion by human buccal mucosa fibroblasts which is excited by lipopolysaccharide. Glycyrrhizin has an important role in the treatment of oral lichen planus.