山莨菪碱对肺缺血一再灌注损伤的保护作用

目的　观察幼猪肺缺血—再灌注过程中 ,肺静脉血肿瘤坏死因子 (TNF -α)的含量变化 ,探讨山莨菪碱 (65 4-2 )对缺血—再灌注肺损伤的保护作用及可能机理。方法　取 45± 4日龄幼猪 12只 ,随机分成 2组 (n =6) :对照组、65 4-2组 ,于阻断右肺动脉前、阻断后 60、90min开放、开放后 60、90、12 0min等时刻点分别抽取左、右肺静脉血 ,ELISA法检测TNP -α含量。结果　 65 4-2组右肺静脉血中TNF -α含量明显低于对照组 (P <0 .0 1)。结论　 65 4-2对肺缺血—再灌注损伤有保护作用

The protection of anisodamine on pulmonary ischemic-reperfusion injury in piglets

Objective To explore the protection and mechanism of anisodamine (654-2) on ischemic-reperfusion injury in piglets through measuring the content of tumor necrosis factor-α (TNF-α) in pulmonary vein blood during ischemic-reperfusion of piglets. Methods Twelve piglets, 45±4 days, were randomly allocated to two groups (n=6): the control group and the 654-2 group, the left and right pulmonary vein blood were sampled at the points of 60, 90 minutes( then opening) before and after clamping the right pulmonary vein, and 60, 90, 120 minutes after opening, respectively. An ELISA method was used to measure the TNF-α content. Results The blood TNF-α content in right pulmonary vein in 654-2 group was significantly lower than that in the control group (P<0.01). Conclusions 654-2 could protect pulmonary ischemic-reperfusion from injury.