Bartonella adhesin a mediates a proangiogenic host cell response |
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Authors: | Riess Tanja Andersson Siv G E Lupas Andrei Schaller Martin Schäfer Andrea Kyme Pierre Martin Jörg Wälzlein Joo-Hee Ehehalt Urs Lindroos Hillevi Schirle Markus Nordheim Alfred Autenrieth Ingo B Kempf Volkhard A J |
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Institution: | Institut für Medizinische Mikrobiologie und Hygiene, Eberhard-Karls-Universit?t, Elfriede-Aulhorn-Strasse 6, 72076 Tübingen, Germany. |
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Abstract: | Bartonella henselae causes vasculoproliferative disorders in humans. We identified a nonfimbrial adhesin of B. henselae designated as Bartonella adhesin A (BadA). BadA is a 340-kD outer membrane protein encoded by the 9.3-kb badA gene. It has a modular structure and contains domains homologous to the Yersinia enterocolitica nonfimbrial adhesin (Yersinia adhesin A). Expression of BadA was restored in a BadA-deficient transposon mutant by complementation in trans. BadA mediates the binding of B. henselae to extracellular matrix proteins and to endothelial cells, possibly via beta1 integrins, but prevents phagocytosis. Expression of BadA is crucial for activation of hypoxia-inducible factor 1 in host cells by B. henselae and secretion of proangiogenic cytokines (e.g., vascular endothelial growth factor). BadA is immunodominant in B. henselae-infected patients and rodents, indicating that it is expressed during Bartonella infections. Our results suggest that BadA, the largest characterized bacterial protein thus far, is a major pathogenicity factor of B. henselae with a potential role in the induction of vasculoproliferative disorders. |
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Keywords: | pilus endothelial cells HIF-1 VEGF angiogenesis |
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