| 轮状病毒感染致乳鼠肠外损害的实验研究 |
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| 引用本文： | 陈军华,解元元,朱朝敏,刘作义. 轮状病毒感染致乳鼠肠外损害的实验研究[J]. 第三军医大学学报, 2009, 31(15) |
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| 作者姓名： | 陈军华 解元元 朱朝敏 刘作义 |
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| 作者单位： | 重庆医科大学附属儿童医院感染消化科,重庆,400014 |
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| 摘 要： | 目的 探讨轮状病毒(rotavirus,RV)感染引起肠外脏器损害情况及其发病机制.方法 用MA104细胞培养猴RV sA-11经口感染生后7 d昆明小鼠乳鼠,实验组每只经口接种0.1 ml培养物冻融液(3.75×107pfu/ml),对照组经口接种0.1 ml正常对照细胞冻融液.接种后3 d处死乳鼠,光镜下观察脑、肺、心、肝、胰和肾组织病理改变,电镜下观察心肌细胞超微结构改变;以免疫荧光方法检测各器官RV抗原分布情况;以Phalloidin-TRITC对丝状肌动蛋白染色并经软件(Imageproplus5.1,IPP5.1)测量分析以观察心肌组织丝状肌动蛋白含量有无改变;以原位凋亡细胞检测方法检测肝细胞和心肌细胞凋亡情况;以巢式RT-PCR方法检测各器官内RV RNA分布情况.结果 在光镜下脑、肺、胰和肾组织未见明显病变,但可见心肌间质水肿,心肌细胞浊肿,肝细胞空泡变性;电镜下心肌细胞肌丝溶解,线粒体内室扩张,滑面内质网扩张,核周间隙增宽;Phalloidin-TRITC染色未见心肌组织丝状肌动蛋白含量改变;除脑组织外其他器官内见到RV抗原分布,而各器官内均有RV-RNA分布;肝细胞凋亡增加,而心肌细胞未见凋亡.结论 RV可播散到肠外脏器并引起病理损害,但总体上程度较轻.病毒感染导致丝状肌动蛋白解聚及细胞骨架损害、诱导细胞凋亡不仅是肠黏膜损伤的机制,同时也是肠外损害的机制.
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| 关 键 词： | 轮状病毒 感染 乳鼠 肠外损害 |
Extraintestinal lesions caused by rotavirus:An experimental study on suckling mice |
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| CHEN Jun-hua,XIE Yuan-yuan,ZHU Chao-min,LIU Zuo-yi. Extraintestinal lesions caused by rotavirus:An experimental study on suckling mice[J]. Acta Academiae Medicinae Militaris Tertiae, 2009, 31(15) |
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| Authors: | CHEN Jun-hua XIE Yuan-yuan ZHU Chao-min LIU Zuo-yi |
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| Affiliation: | CHEN Jun-hua,XIE Yuan-yuan,ZHU Chao-min,LIU Zuo-yi(Department of Infectious Disease,Children's Hospital,Chongqing Medical University,Chongqing 400014,China) |
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| Abstract: | Objective To study the extraintestinal lesions induced by rotavirus(RV)infection and explore the pathogenesis.Methods Simian rotavirus SA11 was cultured in MA-104 cells.After inoculation,the pathological changes in brain,lung,heart,liver,pancreas and kidney tissues were observed,the RV antigens detected,and the apoptotic cells observed.Besides,we stained the filamentous actin(F-actin)with Phalloidine-TRITC,and then quantified the F-actin amount.Results Several pathological changes,inclusive of myocardial in... |
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| Keywords: | rotavirus infection suckling mouse extraintestinal lesion |
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