微粒蛋白质组学研究进展

目的：比较高效价抗人球（效价≥1：512）蛋白、抗人球蛋白试剂（效价≥1：4）（低效价）与微柱凝胶法在自身免疫性溶血性贫血（AIHA）实验室检测中的优势。方法：首先以微柱凝胶法直抗试验筛选阳性血液标本，然后对上述标本分别用高效价抗人球蛋白（效价≥1：512）、抗人球蛋白试剂（效价≥1：4）做直抗半定量试验，γ中和试验。结果：微柱凝胶法易出现假阳性，抗人球蛋白试剂（效价≥1：4）易出现假阴性。高效价抗人球（效价≥1：512）未发现假阳性，而且凝集梯度明显，易于结果判断分析。结论：高效价抗人球（效价≥1：512）在AIHA直抗试验中，较抗人球蛋白试剂（效价≥1：4）与微柱凝胶法具有结果更加准确的明显优势。

Superior of high titer anti-human globulin in autoimmune hemolytic anemia test

Objective：To compare the advantages of high titer of anti-human globulin,anti-human globulin reagent （low titer） and the micro-column gel in autoimmune hemolytic anemia in the laboratory testing. Method：Positive blood samples were screened firstly by direct anti-microcolumn gel test,and were treated with high titer anti- human globulin（titer≥l ： 512）,anti-human reagents （titer≥l ： 4）semi-quantitative test, γ neutralization test. Result： Microcolumn gel proned to false-positive,anti-human globulin reagents proned to false-negative,high titer anti-human globulin did not find false positive, and agglutination was graded easy to judge the results of analysis. Conclusion.. The high titer of anti-human globulin might have the obvious advantage of more accurate results in direct antiglobulin test in autoimmune hemolytic anemia than the anti-human globulin reagents （low titer） and the micro-gel method.