| 去整合素kistrin干预兔后发性白内障形成中晶状体上皮细胞FAK及ERK基因表达的研究 |
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| 引用本文： | 于美沁,谭少健,陈琼,梁皓,何剑峰,李霞,黄敏丽,陈金卯,陈迎迎.去整合素kistrin干预兔后发性白内障形成中晶状体上皮细胞FAK及ERK基因表达的研究[J].眼科新进展,2011,31(6):504-507. |
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| 作者姓名： | 于美沁 谭少健 陈琼 梁皓 何剑峰 李霞 黄敏丽 陈金卯 陈迎迎 |
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| 作者单位： | 广西医科大学一附院眼科中心,广西壮族自治区南宁市,530021 |
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| 基金项目： | 国家自然科学基金资助，广西自然科学基金资助 |
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| 摘 要： | 目的研究去整合素kistrin对兔后发性白内障模型晶状体上皮细胞中黏着斑激酶(focal adhesion kinases,FAK)mRNA和其下游因子细胞外信号调节激酶(extracellular signal regulated kinase,ERK)mRNA表达的影响,以探讨kistrin降低兔后发性白内障形成的可能分子机制。方法 8只新西兰大白兔均行右眼透明晶状体囊外摘出术(extracapsular lens extraction,ECLE)建立后发性白内障模型。随机将8只术眼分为实验组和对照组(每组4眼),术毕实验组囊袋内注入80μg.L-1kistrin0.2mL,对照组注入等量林格氏液。随机选4眼左眼为空白组。术后14d取残留的晶状体囊袋行RT-PCR检测FAK和ERK mRNA的表达。结果正常兔晶状体上皮细胞中(空白组)FAK和ERKmRNA表达量少,分别为1.476±0.802、0.576±0.035。ECLE术后14d对照组FAK和ERK mRNA表达量分别为6.496±1.501、1.158±0.573,实验组分别为6.060±1.512、1.262±0.329,2组表达量均较空白组显著性升高,差异均有统计学意义(均为P<0.05),实验组与对照组比较差异均无统计学意义(均为P>0.05)。结论兔后发性白内障形成的早期,FAK及ERK基因表达上调。应用kistrin未改变此两基因在转录水平上的表达。
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| 关 键 词： | 去整合素 黏着斑激酶 细胞外信号调节激酶 基因表达 信号转导通路 |
Gene expressions of FAK and ERK in lens epithelial cells on rabbit posterior capsular opacification models with kistrin interference |
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| YU Mei-Qin,TAN Shao-Jian,CHEN Qiong,LIANG Hao,HE Jian-Feng,LI Xia,HUANG Min-Li,CHEN Jin-Mao,CHEN Ying-Ying.Gene expressions of FAK and ERK in lens epithelial cells on rabbit posterior capsular opacification models with kistrin interference[J].Recent Advances in Ophthalmology,2011,31(6):504-507. |
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| Authors: | YU Mei-Qin TAN Shao-Jian CHEN Qiong LIANG Hao HE Jian-Feng LI Xia HUANG Min-Li CHEN Jin-Mao CHEN Ying-Ying |
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| Institution: | YU Mei-Qin,TAN Shao-Jian,CHEN Qiong,LIANG Hao,HE Jian-Feng,LI Xia,HUANG Min-Li,CHEN Jin-Mao,CHEN Ying-Ying From the Department of Ophthalmology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China |
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| Abstract: | Objective To evaluate the gene expressions of focal adhesion kinases(FAK)mRNA and extracellular signal regulated kinase(ERK)mRNA in the lens epithelial cells on the rabbit posterior capsular opacification(PCO)models with kistrin interference and investigate the possible mechanisms of this procedure.MethodsExtracapsular lens extraction was performed on right eyes of 8 New Zealand white rabbits to establish the PCO model.Eight right eyes were randomly divided into control group and experimental group,4 eyes i... |
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| Keywords: | disintergrin focal adhesion kinases extracellular signal regulated kinase gene expression signal transduction pathway |
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