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| 小檗碱诱导人胃癌细胞BGC-823细胞凋亡 | |
| 引用本文： | 李国英,杨林西. 小檗碱诱导人胃癌细胞BGC-823细胞凋亡[J]. 中国现代医学杂志, 2004, 14(9): 54-56 |
| 作者姓名： | 李国英 杨林西 |
| 作者单位： | 1. 甘肃省红十字血液中心,甘肃,兰州730046 2. 兰州医学院,生物化学与分子生物学教研室,甘肃,兰州,730046 |
| 摘 要： | 目的观察小檗碱对人胃癌细胞BGC-823凋亡的诱导作用,以探讨具抗癌作用机理.方法采用体外细胞培养技术,以lO～40 mg/L小檗碱作用于培养的BGC-823细胞48 h后,进行光镜、琼脂糖凝胶电泳、流式细胞仪和TUNEL法的观察和分析.结果小檗碱作用后,光镜、TUNEL观察到细胞呈现凋亡特征,琼脂糖凝胶电泳显示细胞DNA呈梯状降解,流式细胞仪分析出现典型亚二倍体峰.结论小檗碱可诱导人胃癌细胞BGC-823细胞凋亡,这可能为小檗碱抗癌的机理之一. |
| 关 键 词： | 小檗碱 人胃癌细胞BGC-823 细胞凋亡 |
Apoptosis of human gastric carcinoma BGC- 823 cells induced by berberine |
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| Abstract. Apoptosis of human gastric carcinoma BGC- 823 cells induced by berberine[J]. China Journal of Modern Medicine, 2004, 14(9): 54-56 | |
| Authors: | Abstract |
| Abstract: | Objective: To investigate the mechanism of the antitumor effect of berberine by observing the apoptosis of human gastric carcinoma BGC- 823 cells induced by berberine. Methods:By technique of cell culture in vitro, BGC -823 cells were treated by berberine in 10 ～ 40 mg/L concentration for 48 hours, then observed and analyzed by light microscopy, agarose gel electrophoresis, flow cytometry and Tunnel. Results:The cells showed characters of apoptosis observed by light microscopy and Tunnel. agarose gel elctrophoresis of DNA showed change of DNA ladder'pattern. A typical subdiploid peak before G0/Gl phase was observed by flow cytonetry. Conclusions:The results confirm that the berberine could induce the apoptosis of BGC -823 cells, which may be one of the mechanisms of bcrberine's antitumor effects. |
| Keywords: | berberine human gastric carcinoma BGC -823 cells apoptosis |
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