α-2a-干扰素抑制血管瘤血管内皮细胞增殖的体外实验

 为探讨α-2 a-干扰素治疗血管瘤的作用机制,采用新鲜皮肤血管瘤组织进行血管内皮细胞组织块培养并传代,通过加入α-2 a-干扰素,观察其对血管内皮细胞增殖的影响.结果:当α-2 a-干扰素应用剂量为5×105u/L和1×106u/L时,对血管内皮细胞的增殖有明显抑制作用.应用5×105u/L,培养第12 d后,血管内皮细胞数量和3H-TdR掺入量明显低于对照组.结论:α-2 a-干扰素可能是通过抑制血管瘤血管内皮细胞的增殖,促进血管瘤的退化而发挥治疗作用.

Effect of alpha- 2a -interferon on growth of endothelial cells of hemangiomas in vitro

This study was aimed to elucidate the mechanism of effect of alpha - 2 a - interferon on hemangiomas. Endothelial cells (ECs ) of hemangiomas were cultured and subcultured by tissue culture method. With addition of alpha - 2 a -interferon , its effect on the growth of ECs was observed. The results showed that. alpha -2 a-interferon (5 X L05 u/L , 1 X 10 u/L) had significant inhibitory effects on proliferation of ECs. In the alpha - 2 a -interferon treated group (5 X 10 u/L) ,EC counts and 3H-TdR incorporation were lower than those in the control group at the 12th day. It is suggested that the alpha-2 a -interferon cures the hemangiomas by inhibiting the growth of ECs of them.