|
|||||
|
|
| LC-ESI-MS/MS法测定草棉花瓣提取物中组氨酸的含量 | |
| 引用本文： | 赛力曼·哈得尔,艾则孜·莫合买提. LC-ESI-MS/MS法测定草棉花瓣提取物中组氨酸的含量[J]. 中国药房, 2011, 0(27): 2563-2564 |
| 作者姓名： | 赛力曼·哈得尔 艾则孜·莫合买提 |
| 作者单位： | 新疆医科大学第一附属医院药剂科;新疆食品药品检验所; |
| 摘 要： | 目的:建立测定草棉花瓣提取物中组氨酸含量的方法。方法:采用高效液相色谱-电喷雾串联质谱法。色谱柱为Altima HP HILIC(150mm×2.1mm×3.0μm),流动相为甲醇-0.1%甲酸溶液(10∶90),流速为0.20mL·min-1,柱温为室温;离子源为ESI,正离子扫描,多反应离子监测模式,用于定量分析的离子反应为m/z156.4→m/z110.1。结果:组氨酸检测浓度在0.05135～0.61620μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9995);平均加样回收率为99.8%,RSD=1.5%(n=9)。结论:本方法准确、灵敏度高、重复性好,可用于草棉花瓣提取物的质量控制。 |
| 关 键 词： | 草棉花瓣提取物 组氨酸 高效液相色谱-电喷雾串联质谱法 含量测定 |
Content Determination of Histidine in Gossypium Herbaceum Flos Extract by LC-ESI-MS/MS |
|
| SALIMA Kader AZIZ Muhammat. Content Determination of Histidine in Gossypium Herbaceum Flos Extract by LC-ESI-MS/MS[J]. China Pharmacy, 2011, 0(27): 2563-2564 | |
| Authors: | SALIMA Kader AZIZ Muhammat |
| Affiliation: | SALIMA Kader(Dept.of Pharmacy,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China) AZIZ Muhammat(Xinjiang Institute for Food and Drug Control,Urumqi 830000,China) |
| Abstract: | OBJECTIVE：To establish a method for the content determination of histidine in Gossypium Herbaceum Flos extract.METHODS：HPLC-Electrospray Tandem Mass Spectrometry（HPLC-ETMS） was adopted.The column was Altima HP HILIC（150 mm×2.1 mm×3.0 μm）with mobile phase consisted of methanol-0.1% formic acid solution（10 ：90）at flow rate of 0.20 mL·min-1.The column temperature was room temperature.The analyte was detected using positive ionization by multiple reaction monitoring mode.The m/z of ion reaction for quantity analysis were 156.4 and 110.1.RESULTS：The linear range of histidine was 0.051 35～0.616 20 μg·mL-1（r=0.999 5） with an average recovery of 99.8%（RSD=1.5%,n=9）.CONCLUSION：The method is accurate,sensitive and reproducible for the quality control of Gossypium Herbaceum Flos extract. |
| Keywords: | Gossypium Herbaceum Flos extract Histidine HPLC-Electrospray Tandem Mass Spectrometry Content determination |
| 本文献已被 CNKI 维普 等数据库收录！ | |