rhBMP-2明胶微球的制备及对人牙周膜细胞增殖和碱性磷酸酶活性影响

目的: 观察重组人骨形成蛋白-2(rhBMP-2)明胶微球(rhBMP-2-GMs)对人牙周膜细胞(PDLCs)增殖和碱性磷酸酶活性的影响. 方法: 体外培养人PDLCs,分别用不同浓度的rhBMP-2和rhBMP-2-GMs作用,用四唑盐比色实验(MTT)和酶动力学方法进行观察. 结果: rhBMP-2和rhBMP-2-GMs均可以明显促进人PDLCs增殖、提高碱性磷酸酶(ALP)活性,并呈剂量依赖性,rhBMP-2-GMs较单独应用rhBMP-2的效应更加显著. 结论: rhBMP-2-GMs利用其对rhBMP-2的缓释作用,对人PDLCs增殖和碱性磷酸酶活性的作用效果优于单独应用rhBMP-2.

Preparation of rhBMP-2-loaded gelatin microspheres and biological effects of rhBMP-2-GMs on proliferation and alkaline phosphatase expression of human periodontal ligament cells

AIM: To evaluate the biological effects of recombinant human bone morphogenetic protein-2 gelatin microspheres (rhBMP-2-GMs) on the proliferation and alkaline phosphatase (ALP) expression of human periodontal ligament cells (PDLCs). METHODS: MTT method and enzyme dynamics method were used to observe the proliferation and ALP expression of PDLCs after different doses of rhBMP-2 and rhBMP-2-GMs were added into the culture medium. RESULTS: Both rhBMP-2 and rhBMP-2-GMs promoted the proliferation and increase the ALP activity of PDLCs in a concentration-related manner. Significantly greater proliferation and expression were found in rhBMP-2-GMs group compared with those in rhBMP-2 group. CONCLUSION: The results indicate that rhBMP-2-GMs can significantly promote the proliferation and increase the ALP activity of PDLCs by sustained release.