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| 沙立度胺单独及联合多柔比星对人肝癌细胞株HepG2中生存素表达的影响 | |
| 引用本文： | 韩亚光,崔彦芝,付华斌,曹世堂,陈京莲,何翠. 沙立度胺单独及联合多柔比星对人肝癌细胞株HepG2中生存素表达的影响[J]. 华北国防医药, 2008, 20(5) |
| 作者姓名： | 韩亚光 崔彦芝 付华斌 曹世堂 陈京莲 何翠 |
| 作者单位： | [1]解放军白求恩国际和平医院256临床部内科,河北正定050800 [2]南方医科大学南方医院肿瘤中心,广州510515 |
| 摘 要： | 目的：观察沙立度胺单独及联合多柔比星对人肝癌细胞株HepG2中生存素（Survivin）表达的影响。方法：常规培养人肝癌细胞株HepG2随机分成4组：空白对照组；多柔比星组（1．0mg／L）；沙立度胺组（50、100、200、400mg／mL，4个对半稀释质量浓度）；联合用药组：沙立度胺（200mg／mL）＋多柔比星（1．0mg／L）。分别对HepG2细胞作用12、24、48、72h后，应用免疫组织化学法观察对Survivin表达的影响。结果：沙立度胺单独及联合多柔比星作用于HepG2细胞可下调Survivin表达，单药沙立度胺以200mg／L作用48h效果最为明显，联合用药组下调Survivin表达优于其余各组，差异有统计学意义（P〈0．05）。结论：沙立度胺可下调人肝癌细胞株HepG2Survivin的表达，且与多柔比星有协同作用，可能是沙立度胺抗肿瘤机制之一。 |
| 关 键 词： | 沙立度胺 多柔比星 肝肿瘤 HepG2 生存素 基因表达调控，肿瘤 |
The Effects of Thalidomide Alone and Combined with Doxorubicin on Survivin Expression in Human Hepatoma HepG2 Cell Lines |
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| HAN Ya-guang,CUI Yan-zhi,FU Hua-bin,CAO Shi-tang,CHEN Jing-lian,HE Cui. The Effects of Thalidomide Alone and Combined with Doxorubicin on Survivin Expression in Human Hepatoma HepG2 Cell Lines[J]. Medical Journal of Beijing Military Region, 2008, 20(5) | |
| Authors: | HAN Ya-guang CUI Yan-zhi FU Hua-bin CAO Shi-tang CHEN Jing-lian HE Cui |
| Affiliation: | HAN Ya-guang1,CUI Yan-zhi2,FU Hua-bin1,CAO Shi-tang1,CHEN Jing-lian1,HE Cui1(1.Medical Department,the 256th Clinical Branch,Bethune International Peace Hospital of PLA,Zhengding,Hebei 050800,China,2.Department of Oncosis,Nanfang Hospital,NanFang Medical University,Guangzhou 510515,China) |
| Abstract: | Objective:To investigate the effects of Thalidomide alone and combined with Doxorubicin on Survivin expression in human hepatoma cell lines HepG2 in vitro.Methods:Human hepatoma cell lines HepG2 cultivated by convention,were randomly divided into 4 groups: untreated control group;Doxorubicin group(1.0 mg/L);Thalidomide group(50,100,200,400 mg/mL);drug combination group(Thalidomide 200 mg/mL+Doxorubicin 1.0 mg/L).After HepG2 was treated for 12,24,48,72 hours as described above,the effects on Survivin express... |
| Keywords: | HepG2 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |