2013-2014年北京市顺义区熟肉制品中单核细胞增生李斯特氏菌致病因子检测分析

目的用PCR方法检测熟肉制品中单核细胞增生李斯特氏菌致病因子,为顺义区熟肉食制品中单增李斯特氏菌污染危害风险评估提供依据。方法利用细菌培养法从熟肉制品监测样本中分离单增李斯特氏菌,PCR检测阳性菌株的16sRNA、侵袭蛋白P60、李氏溶血素、迟发型超敏反应蛋白等致病因子。结果180件食品样本共分离出6株单增李斯特氏菌;通过PCR检测致病因子,6株细菌都检出16sRNA和迟发型超敏反应蛋白的目的基因,5株检出李氏溶血素和侵袭蛋白目的基因,各引物检测目的基因的敏感性略有差异。结论顺义区熟肉制品中单核细胞增生李斯特氏菌污染较为严重,83.33%（5/6）菌株携带李氏溶血素、侵袭蛋白P60等致病因子

PCR analysis for virulence genes of Listeria monocytogenes in delicatessen in Shunyi District,Beijing, from 2013 to 2014.

Objective:In this paper, PCR analysis were used to detect the virulence genes of Listeria monocytogenes in delicatessen in order to provide scientific evidence for risk assessment in Shunyi District of Beijing from 2013 to 2014. Methods:Delicatessen was collected randomly from markets, the isolation of Listeria monocytogenes was performed according to the GB/T 4789.30-2010. At the same time, the genes of 16sRNA, hlyA, ipa60, dth-18 were detected in the isolates. Results:A total of 6 Listeria monocytogenes were isolated from 180 samples. The genes of 16sRNA and dth-18 were found in all the isolates and hlyA and ipa60 were found in 5 of them. The sensitivity of these primers was different slightly. Conclusions:The results indicated that the delicatessen in markets of Shunyi District was contaminated by Listeria monocytogenes to a certain extent. 83.33% (5/6) isolates were hlyA⁺/ipa60⁺ strains.