Identification ofMycoplasma hominis,Ureaplasma urealyticum,andChlamydia trachomatis by the polymerase chain reaction |
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| Authors: | M. Yu. Brodskii V. M. Govorun E. M. Khalilov A. V. Zhdanov L. Z. Faizullin G. T. Sukhikh |
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| Affiliation: | (1) Institute of Physicochemical Medicine, Ministry of Health and Medical Industry of the Russian Federation, Moscow;(2) Center of Obstetrics, Gynecology, and Perinatology, Russian Academy of Medical Sciences, Moscow |
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| Abstract: | ![]()
A procedure for identifying chlamydia, mycoplasmas, and ureaplasmas by the polymerase chain reaction is described which uses a system of four primers complementary to fragments of the 16S RNA gene, one of which detects all three microorganisms and the other three are each specific for a particular organism. With this primer system, chlamydia, mycoplasmas, and ureaplasmas are detectable in a single reaction. The procedure may be used for routine diagnoses in diagnostic laboratories. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 120, N o 12, pp. 606–609, December, 1995 Presented by R. V. Petrov, Member of the Russian Academy of Medical Sciences |
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| Keywords: | polymerase chain reaction Mycoplasma hominis Ureaplasma urealyticum Chlamydia trachomatis |
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