Intracellular potentials of cultured rabbit corneal endothelial cells: Response to temperature and ouabain

The intracellular potentials of rabbit corneal endothelial cells grown in culture were measured with KCl-filled glass microelectrodes. In a physiological salt solution at 35°C the potential difference (p.d.) across the endothelial cell membrane was ?59.2 ± 1.8mV. This p.d. was reduced to ?40.5 ± 1.3mV at 23°C. The addition of 10^?6 M ouabain to confluent cell cultures caused the p.d. to be reduced to about ? 49 mV.