大鼠应激时垂体c-fos和催乳素基因表达关系的研究

目的：探讨应激刺激下，垂体是否有ｃ－ｆｏｓ表达及其与催乳素（ＰＲＬ）基因表达的关系。方法：雌性Ｗｉｓｔａｔａｒ大鼠给予禁锢应激６０ｍｉｎ，用原位杂效方法检测应激开始后１５，３０，６０，１２０，１８０ｍｉｎ时ＲＰＬ ｍＲＮＡ水平。结果：基础状态下垂体前叶ｃ－ｆｏｓ有弱表达，在禁应激开始后１５￣３０ｍｉｎ迅速增加达高峰，尔后下降，在１２０￣１８０ｍｉｎ出现第２次表达高峰，ＰＲＬ ｍＲＮＡ水平的改变与ｃ

The Relationship Between c-fos and Prolactin Gene Expression in the Rat Pituitary after Restraint Stress

Objective: Our purpose was to explore if there is c-fos expression in pituitary and the relationship between c-fos and prolactin (PRL) gene expressions after stress. Methods: Wistar female rats were exposed to restraint stress for 60 min and killed at 15, 30, 60, 120, 180 min after stress. In situ hybridization was performed to detect the levels of c-fos and PRL mRNA in the anterior pituitary. Results: There was a low basal level of c-fos expression in the anterior pituitary. A rapid increase was noted from 15 to 30 min after restraint stress, then the expression declined. A second peak appeared at 120 to 180 min after stress. The change of PRL mRNA was parallel with the c-fos expression. Conclusion: c-fos might mediate the activation of PRL gene expression after stress. Immediate-early genes expression in pituitary may be a marker of activation of neuroendocrine system.