c—jun氨基末端激酶与多药耐药相关蛋白1在结直肠癌组织中的表达

目的研究磷酸化c-jun氨基末端激酶（JNK）与多药耐药相关蛋白1（MRP1）在结直肠癌组织中的表达及JNK在结直肠癌耐药中的可能机制。方法通过免疫组织化学方法研究72例结直肠癌和4JD例正常大肠组织中JNK和MRP1蛋白表达及其关系。结果JNK和MRP1蛋白在结直肠癌组织中的阳性表达率分别为47．22％和51．39％，均明显高于在正常大肠组织中的表达。JNK蛋白阳性表达与结直肠癌患者的性别、年龄、肿瘤大小、部位均无明显相关性（P〉0．05），而与肿瘤分化、淋巴结转移、远处转移及Dukes分期显著相关（P〈0．05）。MRP1蛋白阳性表达与结直肠癌患者的性别、年龄、肿瘤大小、部位及肿瘤分化均无明显相关性（P〉0．05），而与淋巴结转移、远处转移及Dukes分期显著相关（P〈0．05）。结论JNK高表达与结直肠癌的发生及其恶性生物学行为相关，其可能通过上调MRP1的表达参与大肠癌化疗耐药的形成。

The expression of JNK and MRP1 in colorectal carcinoma

Objective To investigate the chnicopathogenical significance of c-jun N-terminal kinase(JNK) and multidrug resistance associated protein 1 (MRP1) expression in colorectal cancer. Methods The expression of JNK and MRP1 was detected in a tissue microarray containing 72 spots of colorectal cancer tissue and 40 spots of nor-mal colorectal tissue by immunohistochemisty. Results The positive expression rate of JNK and MRP1 expression was 47.22% and 51.39% respectively in colorectal cancer,significantly higher than that in normal colon tissue. JNK protein expression was not closely related to gender, age, tumor size and location (P>0.05), but closely related to tumor differentiation,lymph node metastasis,distsnt metastasis and Dukes staging (P<0.05). MRP1 protein expres-sion was not closely related to gender,age,tumor size,location and tumor differentiation(P> 0.05), but closely relat-ed to lymph node metastasis, distant metastasis, Dukes staging (P<0.05). Conclusions The JNK expression in cancer is corrlated with maligant biological behavior and may be involved in the chemotherapeutic resistance by upreg-ulating the expression of MRP1.