同型半胱氨酸诱导基因在正常和畸形人胚心脏中的表达

目的 研究同型半胱氨酸诱导基因(HCY-2)在发育不同时期的正常人胚和畸形人胚心脏中的表达，揭示HCY-2表达蛋白在人胚心肌细胞发育和分化中的作用。方法 取正常和先天性畸形人胚心脏，采用免疫组织化学及图像分析技术对心脏内HCY-2表达变化进行定量测定分析。结果 HCY-2在人胚心脏发育整个时期均表达，其表达部位主要在心肌纤维内；先天性畸形人胚心脏中的HCY-2表达增强。结论 HCY-2表达蛋白通过影响心肌细胞的增殖而影响人胚心脏发育过程，其表达异常与先天性畸形的发生密切相关。

THE EXPRESSION OF HOMOCYSTEINE INDUCED GENE IN NORMAL AND ABNORMAL HUMAN EMBRYONIC HEART

Objective To study the expression of homocysteine induced gene(HCY-2) in the normal and abnormal human embryonic heart. To research the roles of HCY-2 expressing protein in the human embryonic ventricular myocardial cell development and differentiation. Methods Immunohistochemistry and the technique of picture assay were used to detect HCY-2 expression in the normal and abnormal human embryonic heart. Results HCY-2 expresses during the development of human embryonic heart and mainly in the myocardial cells.The expression increases in the congenital malformation.Conclusion HCY-2 expression protein affects the development of human embryonic heart through affecting the myocardial cells proliferation and the abnormal expression has close relation to the congenital malformation.