结直肠中ING1甲基化的性别不一致性研究

目的:检测结直肠癌中是否存在ING1启动子甲基化,ING1甲基化和性别的关系。方法:用甲基化特异性PCR方法检测62例结直肠癌患者的肿瘤组织、相应的正常黏膜的ING1的甲基化状况。结果:ING1甲基化在62份肿瘤标本中有26例发生了甲基化(41.9%),而在相应的正常组织中只有4例(6.45%)。DukesC、D期患者癌组织的甲基化发生率55.6%明显高于DukesA、B期(23.1%)。在肿瘤组织中ING1甲基化表现出明显的性别和年龄差异(P<0.05)。结论:结直肠癌中ING1的甲基化不仅和肿瘤的分期有关,而且可由年龄与性别相关的因素调节,可能是结直肠癌发生过程中的早期事件。

Sexual Discordant Patterns of ING1 Promoter Methylation in Colorectal Cancer

Objective:To investigate the methylation of ING1 promoter in primary colorectal cancer and to assess the sexual discordant patterns of ING1 methylation in colorectal cancer. Methods:We analyzed ING1 methylation in tumor tissues, paired normal tissue from 62 colorectal cancer patients and their clinical characteristics. The methylation status of ING1 was determined by methylation-specific polymerase chain reaction.Results:ING1 methylation was detected in 26 (41.9%) of 62 tissue samples and in 4 (6.45%) of 62 matched normal tissues. The incidence of hypermethylaiotn of ING1(55.6%) in the cancerous tissues of the patients in DukesC and D was significantly higher than that in Dukes A and B(6.45%). ING1 methylation showed an age and gender bias in the tumor tissues(P<0.05).Conclusion:ING1 methylation not only may correlate significantly with the progression of colorectal cancer, but also occurs at the early stage of colorectal cancer and may be regulated by age- and gender-specific factor.