白头翁寒热药性的细胞评价

目的通过实验室前期建立的中药寒热药性的细胞评价方法评价中药白头翁的寒热药性。方法用噻唑蓝（MTT）比色法考察白头翁对HeLa细胞和SGC-7901细胞体外增殖的影响,倒置显微镜观察白头翁对细胞形态特征的影响,台盼蓝染色法分析白头翁的细胞毒作用。结果白头翁在所选质量浓度10~400μg/mL内抑制细胞增殖。形态学观察表明白头翁能使细胞密度减小,细胞固缩变圆,颗粒感增加。台盼蓝染色表明白头翁对这两种细胞没有细胞毒作用。结论白头翁性寒,对所用细胞没有细胞毒作用。

Evaluation of cold-heat characteristics of Pulsatillae Radix

Objective To evaluate the cold-heat characteristics of Pulsatillae Radix by the method we have set up.Methods MTT assay was used to investigate the effect of Pulsatillae Radix on the proliferation of HeLa and SGC-7901 cells in vitro.The morphological changes of HeLa and SGC-7901 cells treated with Pulsatillae Radix were observed through inverted microscope.Trypan-blue staining was used to analyze the cytotoxicity of Pulsatillae Radix.Results Pulsatillae Radix could inhibit the proliferation of HeLa and SGC-7901 cells in the concentration range of 10—400 μg/mL.Morphological observation showed Pulsatillae Radix in the concentration range we have chosen could decrease the density of HeLa and SGC-7901 cells and condense the cells.Trypan-blue staining showed Pulsatillae Radix had no cytotoxicity on HeLa and SGC-7901 cells.Conclusion Pulsatillae Radix is a cold drug and has no cytotoxicity.