| 舍蝇抗菌肽的提取及其对肿瘤细胞生长的抑制作用 |
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| 引用本文： | 邱晓燕,刘艳,陈小麟,陈奕欣.舍蝇抗菌肽的提取及其对肿瘤细胞生长的抑制作用[J].中华卫生杀虫药械,2003,9(1):13-16. |
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| 作者姓名： | 邱晓燕 刘艳 陈小麟 陈奕欣 |
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| 作者单位： | 1. 集美大学生物工程学院,厦门,361000
2. 厦门大学生命科学院,厦门,361005 |
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| 基金项目： | 高等学校重点实验室访问学者基金资助项目 |
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| 摘 要： | 目的：研究含蝇抗菌肽的提取对肿瘤细胞生长的抑制作用；方法：对舍蝇幼虫采用针刺损伤及金黄色葡萄球菌感染诱导方法，并通过研磨、加热、阳离子交换柱层析及Sephadex G—50葡聚糖凝胶柱层析等步骤提取抗菌肽；结果：经SDS—PAGE电泳分离到2条带，分子量分别为4kD，9．6kD；结论：提取的舍蝇抗菌肽能有效地抑制人胃癌细胞MGC80—3和BGC—823，人乳腺癌细胞MCF—7及人肺癌细胞SPC—A—1的体外增殖，并且呈浓度依赖关系。
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| 关 键 词： | 舍蝇抗菌肽 提取 肿瘤细胞生长 抑制作用 抗癌活性 |
| 修稿时间： | 2002年2月9日 |
Anticancer Activity of Antimicrobial Peptides Isolated from Musca Domestica Vicina |
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| QIU Xiao-yan,LIU Yan,CHEN Xiao-lin,CHEN Yi-xin.Anticancer Activity of Antimicrobial Peptides Isolated from Musca Domestica Vicina[J].Chinese Journal of Hygienic Insecticides and Equipments,2003,9(1):13-16. |
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| Authors: | QIU Xiao-yan LIU Yan CHEN Xiao-lin CHEN Yi-xin |
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| Abstract: | Objective:To research the anticancer activity of antimicrobial peptides isolated from Musca domestica vicina.Methods:In this research, two antimicrobial peptides were detected in the extraction of the Musca domestica vicina larvae when the larvae were injured with a needle and then infected with Staphylococcus auerus. These peptides were isolated by a four-step of protocol including grinding, heating, CM-cellulose cation-exchange chromatography, and Sephadex G-50 gel filtration.Results:The molecular weights of these peptides were 4kD, 9.6kD respectively.Conclusion:Musca domestica vicina can inhibit the proliferation of stomach cancer cells MGC80-3 and BGC-823, breast cancer cells MCF-7 and lung cancer cells SPC-A-1 in vitro. |
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| Keywords: | Musca domestica vicina anticancer antimicrobial peptides |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
