KPC-4 Is Encoded within a Truncated Tn4401 in an IncL/M Plasmid,pNE1280, Isolated from Enterobacter cloacae and Serratia marcescens |
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| Authors: | Kendall A Bryant Trevor C Van Schooneveld Ishwor Thapa Dhundy Bastola Laurina O Williams Thomas J Safranek Steven H Hinrichs Mark E Rupp Paul D Fey |
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| Institution: | aDepartments of Pathology and Microbiology;bInternal Medicine, University of Nebraska Medical Center, Omaha, Nebraska, USA;cCollege of Information Science and Technology, University of Nebraska at Omaha, Omaha, Nebraska, USA;dDivision of Laboratory Science and Standards, Centers for Disease Control and Prevention, Atlanta, Georgia, USA;eNebraska Department of Health and Human Services, Lincoln, Nebraska, USA |
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| Abstract: | We describe the transfer of blaKPC-4 from Enterobacter cloacae to Serratia marcescens in a single patient. DNA sequencing revealed that KPC-4 was encoded on an IncL/M plasmid, pNE1280, closely related to pCTX-M360. Further analysis found that KPC-4 was encoded within a novel Tn4401 element (Tn4401f) containing a truncated tnpA and lacking tnpR, ISKpn7 left, and Tn4401 IRL-1, which are conserved in other Tn4401 transposons. This study highlights the continued evolution of Tn4401 transposons and movement to multiple plasmid backbones that results in acquisition by multiple species of Gram-negative bacilli. |
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