保肝消石胶囊的体外溶石、抑菌、解痉作用研究

目的:研究保肝消石胶囊的体外溶石、抑菌、解痉作用。方法:采用体外试验方法,观察保肝消石胶囊等药物对人胆色素结石、胆固醇结石、细菌和兔十二指肠平滑肌的影响。结果:浓度分别为7.568、15.135、30.270g(生药)/ml的保肝消石胶囊对人胆色素结石的溶石重量依次为5.4、8.3、14.7mg,对胆固醇结石的溶石重量依次为2.9、5.1、7.1mg,均有明显的体外溶解作用;对金黄色葡萄球菌、肠球菌、类白喉杆菌和溶血性链球菌有较强的抑制和杀灭作用,相应的最低抑菌浓度(MIC)依次为23.65、23.65、23.65、47.297mg(生药)/ml,最低杀菌浓度(MBC)均为47.297mg(生药)/ml;对大肠埃希菌、伤寒沙门菌及肺炎克雷伯菌均有一定的抑制和杀灭作用,MIC、MBC分别为378.375、756.75mg(生药)/ml;浓度为5.3414mg(生药)/ml的保肝消石胶囊能明显抑制乙酰胆碱所致肠肌收缩的平均张力增强、频率加快和活动度增加(P<0.01);浓度为3.5610、5.3414、7.1219mg(生药)/ml的保肝消石胶囊均能明显抑制氯化钡所致肠肌收缩的平均张力增强、频率加快和活动度增加(P<0.05或P<0.01)。结论:保肝消石胶囊具有一定的体外溶石、抑菌及解痉作用。

The Litholytic,Bacteriostatic and Spasmolytic Effects of Baoganxiaoshi Capsule In Vitro

OBJECTIVE:To study the litholytic,bacteriostatic and spasmolytic effects of Baoganxiaoshi capsule(BXC) in vitro.METHODS:Observed the effects of BXC on human bile pigmental and cholesterol gallstone, bacteria, duodenum smooth muscle of rabbits in vitro.RESULTS:The litholytic concentration of 7.568,15.135 and 30.270g(crude drug)/ml solutions of BXC on human bile pigmental gallstone were 5.4mg,8.3 mg and 14.7 mg respectively,and on human bile cholesterol gallstone were 2.9mg,5.1 mg and 7.1 mg respectively.So BXC have the litholytic function of bile pigmental and cholesterol gallstone.It can strongly inhibit or sterilize staphylococcus aureus, enterococcus, diphtherial bacillus, hemo1ytic streptococcus, and the MICs were 23.65mg(crude drug)/ml ,23.65mg(crude drug)/ml,23.65mg(crude drug)/ml and 47.297mg(crude drug)/ml respectively, and all of the MBCs were 47.297mg(crude drug)/ml.It can inhibit or sterilize coliform,salmonella and pneumococcus, and all of the MICs were 378.375mg(crude drug)/ml, and all of the MBCs were 756.75mg(crude drug)/ml.5.3 414mg(crude drug)/ml solutions of BXC could obviously inhibit the average tension amplified,the frequency accelerated,and the activity increaced,on the duodenum smooth muscle induced by ach(P<0.01).3.5 610mg(crude drug)/ml,5.3 414mg(crude drug)/ml,7.1 219mg(crude drug)/ml solutions of BXC could obviously inhibit the average tension amplified,the frequency accelerated,and the activity increaced,on the duodenum smooth muscle induced by BaCl2(P<0.05 or P<0.01).CONCLUSION: BXC have the litholytic,bacteriostatic and spasmolytic effects in vitro.