三磷酸腺苷氯化镁对肺癌细胞(A549)端粒酶活性的影响

目的:探讨三磷酸腺苷氯化镁(ATP-MgCl2)对肺腺癌细胞的影响及其作用机理.方法:采用连续细胞记数方法观测ATP-MgCl2对A549肺腺癌细胞生长的抑制情况,应用端粒重复序列扩增法(TRAP)观察ATP-MgCl2对癌细胞端粒酶活性的影响,并与ATP作对比.结果:ATP-MgCl2显著抑制癌细胞生长(P＜0.01),且使大量癌细胞死亡,而加药后前两天ATP-MgCl2的抑制、杀伤癌细胞程度较ATP明显(P＜0.05).ATP-MgCl2组和ATP组的端粒酶阳性表达率明显低于对照组(P＜0.01),而前两者间无明显差异(P＜0.05).结论:ATP-MgCl2对肺腺癌细胞有抑制、杀伤(细胞毒)作用.其抗癌作用机理与ATP相似,与抑制癌细胞端粒酶活性有关.MgCl2与ATP结合形成一种活性复合物,增强了ATP的抗癌作用,ATP-MgCl2具有较好的临床应用价值.

Research of Anti-cancer Mechanism of Adenosine Triphosphate Magnesium Chloride on Lung Adenocarcinoma Cell

Objective: To investigate the anticancer function and mechanism of adenosine triphosphate magnesium chloride(ATP-MgCl2)on lung adenocarcinoma cell. Methods: Compared with ATP, inhibiting rate of lung adenocarcinoma cell line(A549) was measured by cell counting and telomerase activity of the cancer cell line was measured by Telomeric Repeat Amplification Protocol (TRAP) assay after operated by ATP-MgCl2. Results: The growth of cancer cells was inhibited markedly by ATP-MgCl2(P<0.01), lots of cells were killed ultimately. ATP-MgCl2 showed higher growth inhibition rate and cytotoxic activity than ATP in the first two days(P<0.05). Positivities for telomerase activity in ATP-MgCl2 and ATP groups were significantly lower than the control group(P<0.01), but there was no marked difference between ATP-MgCl2 and ATP groups(P>0.05). Conclusion: According to these results, we think ATP-MgCl2 shows a marked inhibitory and cytotoxic activity in a human adenocarcinoma cell line (A549). Its anticancer mechanism is similar to ATP, is relevant to inhibiting the activity of telomerase in cancer cell. But because MgCl2 can form an active compound with ATP, which increases the anticancer function of ATP, ATP-MgCl2 has a preferable clinical value.