三氧化二砷治疗Scid小鼠鼻咽癌移植瘤的初步实验研究

目的 :研究三氧化二砷 (As2 O3)对人鼻咽癌小鼠移植瘤的抑制作用。方法 :以人鼻咽癌细胞株CSNE 1为研究对象 ,观察腹腔注射As2 O3对鼻咽癌在Scid小鼠体内生长的抑制作用及其毒副反应。结果 :As2 O3腹腔注射 1mg/kg和 5mg/kg均能在小鼠体内诱导鼻咽癌细胞凋亡。在 5mg/kg剂量组 ,凋亡诱导最明显且能诱导鼻咽癌细胞分化 ,并有显著的抑制肿瘤生长作用 ,抑瘤率为 70 %。上述剂量对小鼠外周血白细胞无抑制作用 ,但病理组织学检查显示对肝脏和心脏有轻度毒性。 10mg/kg迅速致实验鼠中毒死亡。结论 :砷剂在Scid小鼠体内治疗人鼻咽癌移植瘤有效 ,但存在最适剂量问题 ,诱导调亡和分化可能是其抑瘤机制

Inhibition of Arsenic Trioxide on Human Nasopharyngeal Cancer Cells in Scil Mice in Vivo

Objectives To explore the efficacy of Arsenic trioxide(As 2O 3)in the treatment for transplantable human nasopharyngeal carcinoma in Scid mouse.Methods Human nasopharyngeal carcer cells from CSNE 1 cell line were transplanted subcutaneously to Scid mice to produce tumor.The tumor inhibition rate in vivo was observed after intraperitoneally administering of As 2O 3.The histopathologic change in the tumor tissue,and the toxicity of As 2O 3 to the liver,cardiac and white blood count of the host mice were also investigated.Results At the dose of 5 mg/kg and 1 mg/kg,As 2O 3 induced apoptosis in nasopharyngeal carcinoma cells.As 2O 3 of 5 mg/kg also induced the cancer cell differentiation and inhibited gross tumor growth,with the inhibition rate of 70%.No leukopenia was observed at these dose levels but some pathologic changes in liver and cardiac tissue were recognized.It is lethal to the Scid mice at the dose of 10 mg/kg.Conclusions As 2O 3 may have a potential therapeutic effect on nasopharyngeal carcinoma at suitable dosage.The mechanism of antitumor activity may be due,at least in part,to the induction of apoptosis and differentiation in cancer cell.