产生物膜的肺炎克雷伯菌的体外药敏试验探讨

目的探讨产生物膜的肺炎克雷伯菌体外药敏试验的不同,为临床治疗提供准确的药敏参考。方法对临床分离筛选的16株产生物膜的肺炎克雷伯菌用K-B纸片扩散法进行药敏试验,记录24、48、72h这几个时间段的结果。然后将菌株传种数代至其不产生物膜,再行上述操作。最后对两次的药敏结果进行对比分析。结果产生物膜的肺炎克雷伯菌24h抑菌圈的毫米数与48、72h结果比较差异有统计学意义(P0.05)。结论产生物膜的肺炎克雷伯菌用K-B纸片扩散法进行体外药敏试验时,结果宜在48h后报告,这对于该菌的预防具有一定参考价值。

Study on in vitro drug susceptibility test of biomembrane-producing Klebsiella pneumoniae

Objective To study the difference of in vitro drug susceptibility test of biomembrane-producing Klebsiella pneumoni-ae to provide accurate drug reference for clinical treatment.Methods The drug susceptibility test was carried out in clinically isola-ted and screened16 strains of biomembrane-producing Klebsiella pneumoniae by K-B paper diffusion method ,and the results at 24 , 48 ,72 h were recorded.Then the strains were reproduced for several generations until not producing biofilm.Then the above opera-tions were taken again.Finally ,the results of twice drug susceptibility tests were performed the contrastive analysis.Results The mm number of inhibition zone of biomembrane-producing Klebsiella pneumoniae at 24 h had statistical difference compared with that at 48 ,72 h(P<0.05).Conclusion When the in vitro drug susceptibility test of biomembrane-producing Klebsiella pneumoniae is conducted by K-B paper diffusion method ,it is suitable to report the results after 48 h ,this has a certain reference value for pre-venting this bacterium.