高危型HPVDNA联合TCT检测在宫颈癌初筛中的应用价值

目的分析高危型人乳头瘤病毒(HPV)DNA及TCT检测在宫颈癌筛查中的应用价值,评价两者联合检测的临床意义。方法选择2016年3月到2016年5月于该院进行宫颈癌筛查患者402例,分别进行高危型HPV DNA及宫颈液基薄层细胞学检查(TCT)检测,并对怀疑存在宫颈病变的患者行病理组织学检查,依照病理学结果对高危型HPV DNA及TCT的联合检测效果进行比较分析。结果女性高危型HPV DNA检测的阳性率为27.1%(109/402);46例女性TCT检测出现非正常及良性炎症反应,阳性率为11.4%(46/402)。对怀疑存在宫颈恶性病变的123例女性行组织病理学检查,发现32.5%(40/12)女性出现CINⅠ级以上的病变;高危型HPV DNA及TCT联合检测的灵敏度高于单独检测,差异有统计学意义(P0.05);高危型HPV DNA及TCT联合检测的特异度高于高危型HPV DNA单项检测(P0.05),与TCT单项检测比较差异无统计学意义(P0.05)。结论高危型HPV DNA和TCT的检测是宫颈癌筛查的较好方法,高危型HPV DNA和TCT的联合检测可提高宫颈癌前病变的检出率。

Application value of high risk HPV DNA combined with TCT determination in preliminary screening of cervical cancer

Objective To analyze the application value of high risk human papillomavirus (HR HPV) DNA and thinprep cytolo-gy test(TCT ) detection in screening cervical cancer and to evaluate the clinical significance of their combined detection .Methods A total of 402 cases conducting cervical cancer screening in our hospital from March to May 2016 were selected and performed the HR HPV DNA and TCT detection .Those of suspected cervical lesions were performed the histopathological examination .Then the effects of HR HPV DNA and TCT combined detection were compared according to the pathological results .Results The detection positive rate of female HR HPV DNA was 27 .1% (109/402);abnormal and benign inflammation reaction were detected out in 46 cases of female TCT ,the positive rate was 11 .4% (46/402);in 123 cases of suspected cervical malignant lesion ,32 .5% females(40/123) appeared CIN Ⅰgrade or more lesions by histopathological examination ;the sensitivity of HR HPV DNA and TCT combined detection was higher than that of single detection ,the difference was statistically significant (P<0 .05);the specificity of HR HPV DNA and TCT combined detection was higher than that of HR HPV DNA single detection ,the difference was statistically signifi-cant(P<0 .05) ,but the difference was not statistically significant compared with TCT single detection (P>0 .05) .Conclusion The detection of HR HPV DNA and TCT is a good method for screening of cervical cancer ,the combined detection of HR HPV DNA and TCT can improve the detection rate of cervical precancerous lesion .