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| 硫辛酸对老年大鼠肾缺血再灌注后肺损伤时黏附分子ICAM-1表达的影响 | |
| 引用本文： | 张伟,王银环,李巍伟,张一兵,王艳蕾. 硫辛酸对老年大鼠肾缺血再灌注后肺损伤时黏附分子ICAM-1表达的影响[J]. 医学争鸣, 2009, 30(10): 923-925 |
| 作者姓名： | 张伟 王银环 李巍伟 张一兵 王艳蕾 |
| 作者单位： | 华北煤炭医学院,生理教研室,河北,唐山,063000;华北煤炭医学院,机能实验室,河北,唐山,063000;华北煤炭医学院,生化教研室,河北,唐山,063000 |
| 摘 要： | 目的：探讨硫辛酸对老年大鼠肾缺血再灌注致肺损伤时黏附分子ICAM-1（Intercellular adhesion molecule-1）表达的影响.方法：复制大鼠肾缺血再灌注损伤（renal ischemia reperfusion injury,RIRI）模型,采用流式细胞技术、逆转录聚合酶链反应（RT-PCR）和Western Blot观察RIRI发生过程中,血液中CD18阳性的多形核白细胞（polymorphonuclear leukocyte,PMN）百分率、肺系数（pulmonary index,LI）与肺通透指数（pulmonary permeation index,LPI）、肺组织形态学的改变、肺组织ICAM-1在mRNA和蛋白水平的变化及硫辛酸对上述指标的影响.结果：大鼠肾缺血再灌注后LI,LPI及CD18阳性细胞数均明显增加,肺组织髓过氧化物酶（myeloperoxidase,MPO）活性增加,ICAM-1表达明显升高,而提前给予硫辛酸可以明显抑制这些变化,从而对肺起一定保护作用.结论：肺组织细胞ICAM-1表达上调及血液中CD18阳性细胞数的增加可能参与RIRI时肺损伤的发生;硫辛酸可减少血液中CD18阳性细胞数,并且下调肺组织ICAM-1的表达,从而减轻肺损伤. |
| 关 键 词： | 肺损伤 再灌注损伤 硫辛酸 细胞间黏附分子-1 |
Effect of lipoic acid on expression of ICAM-1 of lung tissue following renal ischemia reperfusion in aged rats |
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| ZHANG Wei,WANG Yin-Huan,LI Wei-Wei,ZHANG Yi-Bing,WANG Yan-Lei. Effect of lipoic acid on expression of ICAM-1 of lung tissue following renal ischemia reperfusion in aged rats[J]. Negative, 2009, 30(10): 923-925 | |
| Authors: | ZHANG Wei WANG Yin-Huan LI Wei-Wei ZHANG Yi-Bing WANG Yan-Lei |
| Affiliation: | ZHANG Wei1,WANG Yin-Huan2,LI Wei-Wei3,ZHANG Yi-Bing1,WANG Yan-Lei11Department of Physiology,2Laboratory of Function,3Department of Biochemistry,North Coal Medical College,Tangshan 063000,China |
| Abstract: | AIM:To investigate the influence of lipoic acid on the expression of ICAM-1 of lung tissue following renal ischemia reperfusion in rats. METHODS: The model of renal ischemia reperfusion injury was duplicated. The percentage of positive CD18 polymorphonuclear leucocytes (PMN) in blood, pulmonary index (LI), pulmonary permeation index (LPI), morphological change of lung, changes of mRNA and protein levels were observed by Flow cytometer, RT-PCR and Western blotting. RESULTS: After renal ischemia reperfusion, ... |
| Keywords: | lung/injury reperfusion injury thioctic acid intercellular adhesion molecule-1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |