草乌甲素对小鼠腹腔巨噬细胞趋化功能的影响

目的研究草乌甲素（BLA）对小鼠腹腔巨噬细胞趋化功能的影响。方法小鼠腹腔巨噬细胞经分离后,制成细胞密度为1×10^6个·mL^-1的细胞悬液;而趋化液由4部分按不同比例组成,即细胞培养基RPMI-1640液,溶剂（0.01%DMSO）,BLA溶液（质量浓度分别为80、40、20、10、5μg·L^-1）和趋化因子（MCP-1或补体激活产物）。应用transwell 24孔趋化板检测腹腔巨噬细胞在不同趋化液中的趋化功能,以评价BLA对不同条件下细胞趋化作用的影响。结果 MCP-1和补体激活产物均可使腹腔巨噬细胞的趋化指数显著高于溶剂对照组（P〈0.01）,而该趋化指数可受到BLA溶液的抑制（P〈0.01）。但单用BLA（80、40、20、10、5μg·L^-1）对腹腔巨噬细胞的趋化指数与溶剂对照组相似,差异均无统计学意义（P〉0.05）。结论 BLA能抑制趋化因子对腹腔巨噬细胞的趋化作用,但对腹腔巨噬细胞无直接作用。

Effect of bulleyaconitin A on the chemotaxis of mouse peritoneal macrophages

AIM To determine the effect of bulleyaconitin A（BLA） on the chemotaxis of mouse peritoneal macrophages.METHODS Peritoneal macrophages were isolated from adult male mice.The main groups were as follows： blank control group,MCP-1 group and complement activated product group.Each group contained some subgroups which treated with solvent and BLA（80,40,20,10 and 5μg·L^-1）.The chemotaxis of peritoneal macrophage was assayed by using 24 well transwell permeable supports.RESULTS Peritoneal macrophages treated with MCP-1 or complement activated product showed a greater chemotaxis index than blank control group（P〈0.01）;BLA（80,40,20,10 and 5μg·L^-1） given alone exhibited no significant effect on the chemotaxis（P〉0.05）.However,BLA（80,40,20,10 and 5μg·L^-1） inhibited the increase of chemotaxis index induced by MCP-1 or complement activated product（P〈0.01）.CONCLUSION BLA could inhibit the chemotaxis of macrophages induced by MCP-1 or complement activated product.However,BLA has no significant effect on chemotactic function of peritoneal macrophages in the absence of chemoattractant.