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| 亲和素桥连构建携抗P选择素单抗靶向超声微泡及体外荧光鉴定 | |
| 引用本文： | 郑道文,杨莉,宾建平,陈楚弟,陈少敏,王月刚,吴平生. 亲和素桥连构建携抗P选择素单抗靶向超声微泡及体外荧光鉴定[J]. 中国医学影像技术, 2008, 24(8): 1182-1185 |
| 作者姓名： | 郑道文 杨莉 宾建平 陈楚弟 陈少敏 王月刚 吴平生 |
| 作者单位： | 1. 南方医科大学南方医院心内科,广东,广州,510515 2. 南方医科大学南方医院药学部,广东,广州,510515 3. 南方医科大学南方医院消化科,广东,广州,510515 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 目的 体外荧光方法鉴定生物素-亲和素-生物素(BSB)桥连技术构建携带抗P-选择素单抗靶向超声微泡(MB-BSBp)的町靠性.方法 不同荧光标记物分别标记MB-BSBp的不同部位,观测微泡荧光强度(0、1、2和3级),以普通脂质超声微泡(MB)作对照.结果 加入FITC荧光亲和素孵育,生物紊化脂质微泡(MB-B)呈现明亮的绿色荧光(3级),而MB无荧光显示(0级).以两个浓度梯度(1:4和1:16)的DTAF荧光二抗(抗抗P-选择素单抗抗体)标记MB、MB-B、MB-BS和MB-BSBp,MB-BSBp在两个浓度梯度下均发出明亮的绿色荧光(3级);而MB-B、MB-BS仅在1:4时显示微弱的绿色荧光(1级),MB在两个浓度梯度下均无荧光显示(0级).结论 抗P-选择素单抗通过亲和素桥连有效装配在MB-B表面,体外荧光法是鉴定靶向微泡配体连接可靠性的简便方法. |
| 关 键 词： | P-选择素 靶向超声微泡 荧光 |
| 收稿时间： | 2008-05-26 |
| 修稿时间： | 2008-06-28 |
Construction of targeted ultrasound microbubbles with anti P-selectin monoclone antibody via streptavidin bridge and evaluation of its reliability using fluorescence in vitro |
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| ZHENG Dao-wen,YANG Li,BIN Jian-ping,CHEN Chu-di,CHEN Shao-min,WANG Yue-gang and WU Ping-sheng. Construction of targeted ultrasound microbubbles with anti P-selectin monoclone antibody via streptavidin bridge and evaluation of its reliability using fluorescence in vitro[J]. Chinese Journal of Medical Imaging Technology, 2008, 24(8): 1182-1185 | |
| Authors: | ZHENG Dao-wen YANG Li BIN Jian-ping CHEN Chu-di CHEN Shao-min WANG Yue-gang WU Ping-sheng |
| Affiliation: | Cardiovascular Division,Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;Department of Pharmacy,Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;Cardiovascular Division,Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;Institute for Digestive Diseases,Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;Cardiovascular Division,Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;Cardiovascular Division,Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;Cardiovascular Division,Nanfang Hospital, Southern Medical University, Guangzhou 510515, China |
| Abstract: | |
| Keywords: | P selectin Targeted ultrasound microbubbles Fluorescence |
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