壬基酚对雌性SD大鼠早期性发育的影响

目的 壬基酚是一种典型的环境雌激素,大量应用于塑料、橡胶等行业。本实验旨在探索其对雌性大鼠早期性发育的影响。方法 按照美国NTP(National Toxicology Program)方法对妊娠SD大鼠进行壬基酚口服染毒(0.1%、0.5%和10%).称量F1代出生重、40日龄子宫重,取子宫、卵巢固定,统计初/次级卵泡数,检测细胞增殖核抗原(PCNA)及雌激素受体(ER).结果 染毒组F1代出生重都低于对照组(P均<0.01);40日龄(体重修正)子宫重明显大于对照组(P均<0.01);初级和次级卵泡总数也显着多于对照组(P均<0.01);各组F1代性别比例均为1:1;0.1%和10%壬基酚组卵巢PCNA灰度值明显低于对照组(P<0.01和<0.05);对照组和染毒组子宫ER灰度值差异不明显(P均>0.05).结论 壬基酚能诱导卵泡和子宫的早期发育。

Effects of p-Nonylphenol on Early Sexual Development in Femals SD Rat

Objective Nonylphenol is used widely as plastic additives and surfactants.Recent reports demonstrated alkylphenols are estrogenic.However,the effect of them on animal development remiains unknown.This study is designed to investigate the possible effects of nonylphenol on early development in SD rats.Methods Thirty four pregnant rats are exposured to p-nonylphenol at 0.1%,0.5%,1.0% levels during last ten days of pregnancy.Birth weight,uterus weight of F1 are measured,and morphological characters of uterus and ovary and expression of PCNA and ER are analyzed by histochemistry and immunohistochemistry.Results Nonylphenol at all levels decreased birth weight(P<0.01)and increased uterus weight (P<0.01).Three groups of nonylphenol had more primary and second follicle than the control(P<0.01).The expression of PCNA in granule cell of follicle was enhanced(P<0.05)in 0.1% and 1.0% p-nonylphenol.Conclusion p-nonylphenol can promote early ovary follicle development and increase uterus weight.