| 霍山石斛不同生长阶段遗传稳定性的RAPD分析 |
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| 引用本文： | 刘石泉,李小军,余庆波,谢红,周根余.霍山石斛不同生长阶段遗传稳定性的RAPD分析[J].中国中药杂志,2007,32(10):902-905. |
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| 作者姓名： | 刘石泉 李小军 余庆波 谢红 周根余 |
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| 作者单位： | 1. 湖南城市学院,化学与环境工程系,湖南,益阳,413000
2. 上海师范大学,生命与环境科学学院,生物系遗传学实验室,上海,200234 |
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| 摘 要： | 目的：研究霍山石斛同一蒴果组培不同生长阶段遗传稳定性。方法：利用从20个随机引物筛选的3个稳定性较好的10碱基引物对已继代7～8次的H23种群进行RAPD检测。结果：发现不同阶段内遗传相似系数较大,在85.409 3%～98.360 6%,其中在原球茎、萌芽期和一叶期存在的变异比两叶期、开花期要显著,但程度都极其微弱。结论：来自同一蒴果的变异非常小,建立霍山石斛稳定的无性快繁系是切实可行的。
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| 关 键 词： | 随机扩增多态性DNA 霍山石斛 生长阶段 遗传稳定性 |
| 文章编号： | 1001-5302（2007）10-0902-04 |
| 收稿时间： | 2006-10-20 |
| 修稿时间： | 2006-10-20 |
Analysis on genetic stability in different development stages of Dendrobium huoshanense by RAPD |
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| LIU Shi-quan; LI Xiao-jun; YU Qing-bo; XIE Hong; ZHOU Gen-yu.Analysis on genetic stability in different development stages of Dendrobium huoshanense by RAPD[J].China Journal of Chinese Materia Medica,2007,32(10):902-905. |
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| Authors: | LIU Shi-quan; LI Xiao-jun; YU Qing-bo; XIE Hong; ZHOU Gen-yu |
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| Institution: | 1. Department of Chemistry and Environment Engineering, Hunan City University, Yiyang 413000, China; 2. Genetics Laboratory, Department of Biology, College of Life and Environment Science, Shanghai Normal University, Shanghai 200234, China |
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| Abstract: | Objective: To research the hereditary stability of Dendrobium huoshanense which were subcultured 7-8 times in the same tissue culture system. Method: Using three primers of arbitrary decamer oligonucleotide sequences from 20 primers for DNA amplification, random amplified polymorphic DNA (RAPD) were performed. Result: The genetic similarity coefficient was varied from 85.4% to 98.4%. The variation of protocorm, germination and monoleaf were rather more notable than that of bileaf and inflorescence. Conclusion: The variation of the same pod is quite small. It is feasible to set up the rapid propagation system of D. huoshanense . |
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| Keywords: | random amplified polymorphic DNA(RAPD) Dendrobium huoshanense development stages genetic stability |
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