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| 从人源免疫抗体库筛选抗乙型肝炎病毒PreS1单链抗体 | |
| 引用本文： | 张志超,李琦,胡学军. 从人源免疫抗体库筛选抗乙型肝炎病毒PreS1单链抗体[J]. 中华肝脏病杂志, 2003, 11(2): 90-92 |
| 作者姓名： | 张志超 李琦 胡学军 |
| 作者单位： | 1. 116012,大连理工大学生物工程系 2. 大连,宝生物工程大连公司 |
| 摘 要： | 目的 获得高亲和力的全人源化抗乙型肝炎病毒PreS1的单链抗体。 方法 以健康供血者的外周血淋巴细胞为来源,以PreS1肽体外致敏后,借助噬菌体展示技术构建人源免疫单链抗体库,库容量7×10 8。 结果 以PreS1肽进行3轮固相筛选。获得了亲和力10 7～10 8mol/L的抗PreS1单链抗体。结论 通过体外致敏的方法构建人源免疫抗体库,可以快速获得高亲和力基因工程抗体。 |
| 关 键 词： | 乙型肝炎病毒 PreSl单链抗体 人源免疫抗体库 外周血淋巴细胞 噬菌体展示技术 |
| 修稿时间： | 2001-09-19 |
Generation of high affinity humanized single-chain antibody against PreS1 of hepatitis B virus from immune phage displayed antibody library |
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| ZHANG Zhi-chao,LI Qi,HU Xue-jun. Generation of high affinity humanized single-chain antibody against PreS1 of hepatitis B virus from immune phage displayed antibody library[J]. Chinese journal of hepatology, 2003, 11(2): 90-92 | |
| Authors: | ZHANG Zhi-chao LI Qi HU Xue-jun |
| Affiliation: | Department of Bioengineering, Dalian University of Technology, Dalian 116012, China. |
| Abstract: | OBJECTIVE: Screening and characterizing high affinity completely humanized single-chain antibody (ScFv) against PreS1 of hepatitis B virus. METHODS: A combinatorial library of phage-displayed human ScFv, genes of which were derived from peripheral blood lymphocytes immunized by PreS1 of Hepatitis B Virus in vitro, was constructed. The library contained 7 10(8) clones. RESULTS: After 3 rounds panning, a high affinity (K=10(7) to 10(8) mol/L) ScFv specific to PreS1 was obtained. The V(H) belonged to human V(H4) family, and V(1) to V(4) by sequence analysis. CONCLUSION: This study suggests that the method of antigen stimulation in vitro is an expeditious way for the source of human immune antibody. And the ScFv may provide a more satisfactory therapy. |
| Keywords: | Hepatitis B virus Immune antibody library PreS1 hepatitis B virus |
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