四生汤对照射小鼠胸腺淋巴细胞凋亡和抗氧化能力的作用

目的探讨四生汤对受辐射小鼠胸腺淋巴细胞抗氧化能力及细胞凋亡的影响及作用机制。方法建立60Co（辐射强度为3Gy）对体外培养的小鼠胸腺淋巴细胞辐射损伤模型。实验设计分为5组：对照组,60Co模型组,高、中、低剂量四生汤组。MTT法检测细胞活性;酶生化法测定各组小鼠胸腺细胞胞浆超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量;流式细胞仪测定小鼠胸腺淋巴细胞凋亡率,免疫细胞化学法检测各组Bcl-2和Bax的表达。结果在强度为3Gy60Co辐射情况下,1%-4%浓度范围内四生汤能显著提高小鼠胸腺淋巴细胞的活性;显著提高细胞浆中SOD活性,降低MDA含量;各四生汤组淋巴细胞凋亡率明显低于模型组（P〈0.01）;Bcl-2蛋白表达增强,Bax蛋白表达减弱。结论四生汤能提高体外培养的小鼠胸腺淋巴细胞活性,抑制60Co辐射诱导的淋巴细胞凋亡,其作用机制与四生汤提高抗氧化酶SOD活性,抑制脂质过氧化水平及调节凋亡因子Bcl-2与Bax之间的平衡有关。

Effect of Decoction of Four Fresh Drugs on apoptosis and anti-oxidative ability of irradiated thymocytes in mice

Objective To study the effect of Decoction of Four Fresh Drugs on anti-oxidative ability and apoptosis of irradiated thymocytes in mice and its mechanisms.Methods A mouse model of in vitro cultured thymocyte injury was induced by 60Co at the dose of 3Gy.Thymocytes were divided into normal control group,60Co model group,and low-,medium-and high-dose Decoction of Four Fresh Drugs groups.Cell activity was detected with MTT method.Superoxide dismutase（SOD） activity and malondialdehyde（MDA） level were measured with enzyme biochemical method following irradiation.Apoptosis of lymphocytes was detected by flow cytometry and expressions of bcl-2 and bax protein were detected with immunocytochemical method.Results After the cells were irradiated with 60Co at the dose of 3Gy,the activities of lymphocytes in thymus and SOD in cytoplasm were increased while the MDA level was decreased in mice after treatment with Decoction of Four Fresh Drugs（P〈0.01）.The expression level of bcl-2 was significantly lower while that of Bcl-2 was significantly higher and that of Bax was lower in Decoction of Four Fresh Drugs groups than in 60Co model group（P〈0.01）.Conclusion Decoction of Four Fresh Drugs can increase the activity of in vitro cultured mouse thymocytes,inhibit apoptosis of 60Co-irradiated lymphocytes and improve their anti-oxidative ability by increasing the activity of antioxidant enzymes and regulating the balance between bcl-2 and bax.